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基本情報
登録情報 | データベース: PDB / ID: 1i7x | ||||||
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タイトル | BETA-CATENIN/E-CADHERIN COMPLEX | ||||||
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![]() | CELL ADHESION / E-cadherin / beta-catenin / protein-protein complex / extended interface / armadillo repeat | ||||||
機能・相同性 | ![]() lung cell differentiation / epicardium-derived cardiac vascular smooth muscle cell differentiation / mesenchyme morphogenesis / RUNX3 regulates WNT signaling / Regulation of CDH11 function / cardiac vascular smooth muscle cell differentiation / Beta-catenin phosphorylation cascade / uterine epithelium development / Disassembly of the destruction complex and recruitment of AXIN to the membrane / Apoptotic cleavage of cell adhesion proteins ...lung cell differentiation / epicardium-derived cardiac vascular smooth muscle cell differentiation / mesenchyme morphogenesis / RUNX3 regulates WNT signaling / Regulation of CDH11 function / cardiac vascular smooth muscle cell differentiation / Beta-catenin phosphorylation cascade / uterine epithelium development / Disassembly of the destruction complex and recruitment of AXIN to the membrane / Apoptotic cleavage of cell adhesion proteins / hair cycle process / positive regulation of epithelial cell differentiation / TCF dependent signaling in response to WNT / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / trachea morphogenesis / mesenchyme development / endoderm formation / Formation of the beta-catenin:TCF transactivating complex / VEGFR2 mediated vascular permeability / positive regulation of heparan sulfate proteoglycan biosynthetic process / lung induction / positive regulation of branching involved in lung morphogenesis / cranial ganglion development / renal vesicle formation / renal inner medulla development / renal outer medulla development / nephron tubule formation / mesenchymal stem cell differentiation / beta-catenin-ICAT complex / metanephros morphogenesis / genitalia morphogenesis / embryonic skeletal limb joint morphogenesis / neural plate development / Deactivation of the beta-catenin transactivating complex / glial cell fate determination / regulation of secondary heart field cardioblast proliferation / astrocyte-dopaminergic neuron signaling / negative regulation of mitotic cell cycle, embryonic / canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation / oviduct development / beta-catenin-TCF7L2 complex / regulation of nephron tubule epithelial cell differentiation / negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis / regulation of timing of anagen / regulation of branching involved in salivary gland morphogenesis / Ca2+ pathway / central nervous system vasculogenesis / regulation of epithelial cell differentiation / Schwann cell proliferation / salivary gland cavitation / animal organ development / regulation of centriole-centriole cohesion / glandular epithelial cell differentiation / regulation of protein catabolic process at postsynapse, modulating synaptic transmission / RHO GTPases activate IQGAPs / regulation of centromeric sister chromatid cohesion / Adherens junctions interactions / Degradation of beta-catenin by the destruction complex / embryonic axis specification / ventricular compact myocardium morphogenesis / endodermal cell fate commitment / morphogenesis of embryonic epithelium / Scrib-APC-beta-catenin complex / Degradation of the extracellular matrix / beta-catenin-TCF complex / lens morphogenesis in camera-type eye / positive regulation of fibroblast growth factor receptor signaling pathway / dorsal root ganglion development / synaptic vesicle clustering / positive regulation of cell-cell adhesion / acinar cell differentiation / Integrin cell surface interactions / dorsal/ventral axis specification / proximal/distal pattern formation / neuron fate determination / lateral loop / layer formation in cerebral cortex / positive regulation of myoblast proliferation / positive regulation of endothelial cell differentiation / establishment of blood-retinal barrier / sympathetic ganglion development / fungiform papilla formation / delta-catenin binding / lung epithelial cell differentiation / embryonic foregut morphogenesis / hindbrain development / regulation of calcium ion import / positive regulation of determination of dorsal identity / ectoderm development / positive regulation of skeletal muscle tissue development / regulation of neuron migration / negative regulation of axon extension / cell-cell adhesion mediated by cadherin / cranial skeletal system development / regulation of osteoclast differentiation / regulation of protein localization to cell surface / cellular response to indole-3-methanol / mesenchymal cell proliferation involved in lung development / endothelial tube morphogenesis / positive regulation of odontoblast differentiation 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Huber, A.H. / Weis, W.I. | ||||||
![]() | ![]() タイトル: The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin. 著者: Huber, A.H. / Weis, W.I. #1: ![]() タイトル: The Cadherin Cytoplasmic Domain is Unstructured in the Absence of Beta-Catenin: A Possible Mechanism for Regulating Cadherin Turnover 著者: Huber, A.H. / Stewart, D.B. / Laurents, D.V. / Nelson, W.J. / Weis, W.I. #2: ![]() タイトル: Crystal Structure of a Beta-catenin/Tcf Complex 著者: Graham, T.A. / Weaver, C. / Mao, F. / Kimelman, D. / Xu, W. #3: ![]() タイトル: Three-dimensional Structure of the Armadillo Repeat Region of Beta-catenin 著者: Huber, A.H. / Nelson, W.J. / Weis, W.I. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 211.6 KB | 表示 | ![]() |
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PDB形式 | ![]() | 170.4 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
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アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 58844.117 Da / 分子数: 2 / 断片: ARMADILLO DOMAIN / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() #2: タンパク質 | 分子量: 17004.023 Da / 分子数: 2 / 断片: CYTOPLASMIC DOMAIN / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() #3: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.75 Å3/Da / 溶媒含有率: 67.24 % | ||||||||||||||||||||||||||||||
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7.5 詳細: POLYETHYLENEIMINE TRIS-HCL ISOPROPANOL, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 20 ℃ | ||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC QUANTUM 4 / 検出器: CCD / 日付: 1999年12月23日 詳細: 1) Cylindrical 1m long collimating mirror (Rh-coated Si) before monochromator 2) Toroidal focusing mirror 1.2m long (Rh-coated zerodur) after the monochromator |
放射 | モノクロメーター: Double-Crystal Si 111 crystals / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.033 Å / 相対比: 1 |
反射 | 解像度: 3→30 Å / Num. all: 46268 / Num. obs: 44525 / % possible obs: 98.5 % / Observed criterion σ(I): -3 / 冗長度: 2.5 % / Limit h max: 60 / Limit h min: -60 / Limit k max: 44 / Limit k min: -60 / Limit l max: 31 / Limit l min: 0 / Observed criterion F max: 177611.65 / Observed criterion F min: 0.32 / Rmerge(I) obs: 0.079 / Net I/σ(I): 8.6 |
反射 シェル | 解像度: 3→3.11 Å / Rmerge(I) obs: 0.368 / % possible all: 98.6 |
反射 | *PLUS Num. measured all: 109516 |
反射 シェル | *PLUS % possible obs: 98.6 % |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: Armadillo repeat domain of murine beta-catenin, PDB code 2bct. 解像度: 3→30 Å / Rfactor Rfree error: 0.004 / Occupancy max: 1 / Occupancy min: 1 / Data cutoff high rms absF: 10000 / Isotropic thermal model: Grouped isotropic / 交差検証法: THROUGHOUT / σ(F): 0 / 立体化学のターゲット値: maximum likelihood
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溶媒の処理 | 溶媒モデル: CNS bulk solvent model used / Bsol: 30.8719 Å2 / ksol: 0.286341 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso max: 200.81 Å2 / Biso mean: 59.09 Å2 / Biso min: 2.07 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 3→30 Å
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拘束条件 |
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LS精密化 シェル | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 8
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Xplor file | Serial no: 1 / Param file: protein_rep.param | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ソフトウェア | *PLUS 名称: CNS / バージョン: 1 / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS 最低解像度: 30 Å / σ(F): 0 / % reflection Rfree: 8.1 % / Rfactor all: 0.2 / Rfactor obs: 0.196 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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LS精密化 シェル | *PLUS Rfactor Rfree: 0.315 / % reflection Rfree: 6.6 % / Rfactor Rwork: 0.318 |