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Yorodumi- PDB-1i4b: The solution structure of the major family of the mutant stem loo... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1i4b | ||||||||||||||||||||
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Title | The solution structure of the major family of the mutant stem loop C 5'UUA3' triloop of brome mosaic virus (+) strand RNA | ||||||||||||||||||||
Components | 5'-R(*Keywords | RNA / Triloop / Stem-Loop / Mutant / Replication / BMV | Function / homology | RNA / RNA (> 10) | Function and homology information Method | SOLUTION NMR / simulated annealing | Model type details | minimized average | Authors | Tinoco Jr., I. / Kim, C.-H. | Citation | Journal: J.Mol.Biol. / Year: 2001 Title: Structural and thermodynamic studies on mutant RNA motifs that impair the specificity between a viral replicase and its promoter Authors: Kim, C.-H. / Tinoco Jr., I. #1: Journal: Nat.Struct.Biol. / Year: 2000 Title: RNA motifs that determine specificity between a viral Replicase and its promoter Authors: Kao, C.C. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1i4b.cif.gz | 14.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1i4b.ent.gz | 10.2 KB | Display | PDB format |
PDBx/mmJSON format | 1i4b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i4/1i4b ftp://data.pdbj.org/pub/pdb/validation_reports/i4/1i4b | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 4133.501 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence is a mutant triloop of which wild type sequence is naturally occured in BMV (+) strand RNA |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2mM 5'UUA3' triloop, unlabeled, 5mM Sodium Phosphate buffer, pH 6.5 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 5mM NaCl / pH: 6.5 / Pressure: ambient / Temperature: 283 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |