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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1hzi | ||||||
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タイトル | INTERLEUKIN-4 MUTANT E9A | ||||||
![]() | INTERLEUKIN-4 | ||||||
![]() | CYTOKINE / IL-4 / 4-HELIX-BUNDLE | ||||||
機能・相同性 | ![]() positive regulation of eosinophil chemotaxis / interleukin-4 receptor binding / positive regulation of isotype switching to IgE isotypes / B cell costimulation / negative regulation of complement-dependent cytotoxicity / negative regulation of macrophage activation / negative regulation of chronic inflammatory response / positive regulation of cellular respiration / regulation of isotype switching / Interleukin-18 signaling ...positive regulation of eosinophil chemotaxis / interleukin-4 receptor binding / positive regulation of isotype switching to IgE isotypes / B cell costimulation / negative regulation of complement-dependent cytotoxicity / negative regulation of macrophage activation / negative regulation of chronic inflammatory response / positive regulation of cellular respiration / regulation of isotype switching / Interleukin-18 signaling / negative regulation of neuroinflammatory response / negative regulation of epithelial cell migration / negative regulation of T-helper 17 cell differentiation / dendritic cell differentiation / positive regulation of T-helper 2 cell cytokine production / positive regulation of isotype switching to IgG isotypes / neuroinflammatory response / interleukin-4-mediated signaling pathway / macrophage activation / positive regulation of interleukin-13 production / myeloid dendritic cell differentiation / positive regulation of mast cell degranulation / regulation of phosphorylation / positive regulation of mononuclear cell migration / positive regulation of amyloid-beta clearance / type 2 immune response / activation of Janus kinase activity / positive regulation of MHC class II biosynthetic process / T-helper 2 cell differentiation / negative regulation of cellular response to transforming growth factor beta stimulus / positive regulation of T cell differentiation / positive regulation of myoblast fusion / positive regulation of ATP biosynthetic process / negative regulation of osteoclast differentiation / negative regulation of acute inflammatory response / positive regulation of interleukin-10 production / positive regulation of macroautophagy / negative regulation of tumor necrosis factor production / regulation of immune response / negative regulation of endothelial cell apoptotic process / positive regulation of T cell proliferation / positive regulation of B cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / extrinsic apoptotic signaling pathway in absence of ligand / positive regulation of defense response to virus by host / T cell activation / cholesterol metabolic process / B cell differentiation / innate immune response in mucosa / cytokine activity / negative regulation of extrinsic apoptotic signaling pathway / microglial cell activation / growth factor activity / negative regulation of inflammatory response / positive regulation of receptor-mediated endocytosis / positive regulation of cold-induced thermogenesis / Interleukin-4 and Interleukin-13 signaling / positive regulation of cell migration / immune response / negative regulation of DNA-templated transcription / positive regulation of cell population proliferation / positive regulation of gene expression / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Hulsmeyer, M. / Scheufler, C. / Dreyer, M.K. | ||||||
![]() | ![]() タイトル: Structure of interleukin 4 mutant E9A suggests polar steering in receptor-complex formation. 著者: Hulsmeyer, M. / Scheufler, C. / Dreyer, M.K. #1: ![]() タイトル: A MIXED-CHARGE PAIR IN HUMAN INTERLEUKIN 4 DOMINATES HIGH-AFFINITY INTERACTION WITH THE RECEPTOR A CHAIN 著者: Wang, Y. / Shen, B.-J. / Sebald, W. #2: ![]() タイトル: Crystal Structure of the Interleukin-4/Receptor a chain complex reveals a mosaic binding interface 著者: Hage, T. / Sebald, W. / Reinemer, P. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 43.8 KB | 表示 | ![]() |
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PDB形式 | ![]() | 29.8 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 428.1 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 430.5 KB | 表示 | |
XML形式データ | ![]() | 9.2 KB | 表示 | |
CIF形式データ | ![]() | 12.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 1rcbS S: 精密化の開始モデル |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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詳細 | The biological assembly is a monomer. |
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要素
#1: タンパク質 | 分子量: 14931.213 Da / 分子数: 1 / 変異: E9A / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() | ||
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#2: 化合物 | ChemComp-SO4 / #3: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.15 Å3/Da / 溶媒含有率: 60 % | ||||||||||||||||||||
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.4 詳細: ammonium sulfate, sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||
結晶化 | *PLUS | ||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 1998年6月11日 |
放射 | モノクロメーター: GRAPHITE / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2.05→20 Å / Num. all: 12421 / Num. obs: 12421 / % possible obs: 99.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / 冗長度: 5 % / Biso Wilson estimate: 21.5 Å2 / Rmerge(I) obs: 0.037 / Net I/σ(I): 34.8 |
反射 シェル | 解像度: 2.05→2.1 Å / 冗長度: 4.6 % / Rmerge(I) obs: 0.168 / Mean I/σ(I) obs: 7.7 / Num. unique all: 795 / % possible all: 98.8 |
反射 | *PLUS 最低解像度: 20 Å / % possible obs: 99.2 % / Num. measured all: 61298 / Rmerge(I) obs: 0.034 |
反射 シェル | *PLUS % possible obs: 98.8 % / Num. unique obs: 795 / Num. measured obs: 4531 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 1RCB 解像度: 2.05→19.88 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 1148676.14 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: Engh & Huber
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溶媒の処理 | 溶媒モデル: FLAT MODEL / Bsol: 55.42 Å2 / ksol: 0.377 e/Å3 | ||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 32.5 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.05→19.88 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.05→2.18 Å / Rfactor Rfree error: 0.023 / Total num. of bins used: 6
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Xplor file |
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ソフトウェア | *PLUS 名称: CNS / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS σ(F): 0 / % reflection Rfree: 7.1 % | ||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS Biso mean: 32.5 Å2 | ||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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LS精密化 シェル | *PLUS Rfactor Rfree: 0.285 / % reflection Rfree: 7.7 % / Rfactor Rwork: 0.252 |