Text: This structure was determinined using 3D triple-resonance experiments with the enhanced-sensitivity pulse field gradient approach, simultaneous 15N/13C-edited NOESY and 2D constant time 13C-1H HSQC techniques.
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試料調製
詳細
内容: 1mM sample is the mixture of U-15N/13C labeled holo- (60%)and apo-ACP (40%) 溶媒系: 50 mM Bis-Tris, 100 mM sodium chloride, 10 mM magnesium chloride and 10 mM DTT in 5% D2O, 95% H2O
手法: Distance geometry, Simulated annealing, molecular dynamics ソフトェア番号: 1 詳細: The structures are based on a total of 1050 distance constraints from NOE and H-bond, 96 dihedral angle constraints from Talos program and 76 pairs of CA/CB chemical shift constraints