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Open data
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Basic information
Entry | Database: PDB / ID: 1hww | ||||||
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Title | GOLGI ALPHA-MANNOSIDASE II IN COMPLEX WITH SWAINSONINE | ||||||
![]() | ALPHA-MANNOSIDASE II | ||||||
![]() | HYDROLASE / N-terminal alpha-beta domain / three helix bundle / 2 C-terminal beta barrels | ||||||
Function / homology | ![]() mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase / mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity / rhodopsin biosynthetic process / encapsulation of foreign target / Reactions specific to the complex N-glycan synthesis pathway / mannosidase activity / alpha-mannosidase activity / mannose metabolic process / N-glycan processing / Golgi stack ...mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase / mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity / rhodopsin biosynthetic process / encapsulation of foreign target / Reactions specific to the complex N-glycan synthesis pathway / mannosidase activity / alpha-mannosidase activity / mannose metabolic process / N-glycan processing / Golgi stack / protein deglycosylation / protein glycosylation / carbohydrate binding / lysosome / Golgi membrane / endoplasmic reticulum / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | van den Elsen, J.M.H. / Kuntz, D.A. / Rose, D.R. | ||||||
![]() | ![]() Title: Structure of Golgi alpha-mannosidase II: a target for inhibition of growth and metastasis of cancer cells. Authors: van den Elsen, J.M. / Kuntz, D.A. / Rose, D.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 241.5 KB | Display | ![]() |
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PDB format | ![]() | 186.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 482 KB | Display | ![]() |
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Full document | ![]() | 497.5 KB | Display | |
Data in XML | ![]() | 47.1 KB | Display | |
Data in CIF | ![]() | 71.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1htySC ![]() 1hxkC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The biological assembly is a monomer with one copy of the protein in the asymmetric unit |
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Components
-Protein / Sugars , 2 types, 2 molecules A![](data/chem/img/NAG.gif)
![](data/chem/img/NAG.gif)
#1: Protein | Mass: 116222.789 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q24451, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase |
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#2: Sugar | ChemComp-NAG / |
-Non-polymers , 4 types, 988 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/SWA.gif)
![](data/chem/img/MRD.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/SWA.gif)
![](data/chem/img/MRD.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-ZN / |
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#4: Chemical | ChemComp-SWA / |
#5: Chemical | ChemComp-MRD / ( |
#6: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.8 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 6000, MPD, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 25, 2000 / Details: Osmic focussing optics (mirrors) |
Radiation | Monochromator: Osmic mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54189 Å / Relative weight: 1 |
Reflection | Resolution: 1.87→69.24 Å / Num. all: 87633 / Num. obs: 87633 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.9 % / Biso Wilson estimate: 13.8 Å2 / Limit h max: 36 / Limit h min: 0 / Limit k max: 58 / Limit k min: 0 / Limit l max: 74 / Limit l min: 0 / Observed criterion F max: 338732 / Observed criterion F min: 0.32 / Rmerge(I) obs: 0.078 / Net I/σ(I): 24 |
Reflection shell | Resolution: 1.87→1.91 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.452 / Mean I/σ(I) obs: 3.8 / Num. unique all: 5601 / % possible all: 96.9 |
Reflection | *PLUS Num. all: 87633 / Num. obs: 87386 |
Reflection shell | *PLUS % possible obs: 96.9 % / Num. unique obs: 5601 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1HTY Resolution: 1.87→69.24 Å / Rfactor Rfree error: 0.003 / Occupancy max: 1 / Occupancy min: 1 / Cross valid method: THROUGHOUT / σ(F): 1 / σ(I): 1 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: CNS bulk solvent model used / Bsol: 42.2797 Å2 / ksol: 0.354131 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 64.55 Å2 / Biso mean: 19.44 Å2 / Biso min: 8.18 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.87→69.24 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.87 Å / Lowest resolution: 500 Å / σ(F): 1 / % reflection Rfree: 10 % / Rfactor obs: 0.181 / Rfactor Rfree: 0.209 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.255 / % reflection Rfree: 4.7 % / Rfactor Rwork: 0.225 |