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- PDB-1hq1: STRUCTURAL AND ENERGETIC ANALYSIS OF RNA RECOGNITION BY A UNIVERS... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1hq1 | ||||||
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Title | STRUCTURAL AND ENERGETIC ANALYSIS OF RNA RECOGNITION BY A UNIVERSALLY CONSERVED PROTEIN FROM THE SIGNAL RECOGNITION PARTICLE | ||||||
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![]() | SIGNALING PROTEIN/RNA / PROTEIN-RNA COMPLEX / DOUBLE HELIX / TETRALOOP / INTERNAL LOOP / SIGNAL RECOGNITION PARTICLE / SRP / RIBONUCLEOPROTEIN / SIGNALING PROTEIN-RNA COMPLEX | ||||||
Function / homology | ![]() signal recognition particle / endoplasmic reticulum signal peptide binding / signal-recognition-particle GTPase / SRP-dependent cotranslational protein targeting to membrane / 7S RNA binding / SRP-dependent cotranslational protein targeting to membrane, translocation / protein targeting to membrane / ribonucleoprotein complex / GTPase activity / GTP binding ...signal recognition particle / endoplasmic reticulum signal peptide binding / signal-recognition-particle GTPase / SRP-dependent cotranslational protein targeting to membrane / 7S RNA binding / SRP-dependent cotranslational protein targeting to membrane, translocation / protein targeting to membrane / ribonucleoprotein complex / GTPase activity / GTP binding / ATP hydrolysis activity / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Batey, R.T. / Sagar, M.B. / Doudna, J.A. | ||||||
![]() | ![]() Title: Structural and energetic analysis of RNA recognition by a universally conserved protein from the signal recognition particle. Authors: Batey, R.T. / Sagar, M.B. / Doudna, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 119.3 KB | Display | ![]() |
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PDB format | ![]() | 86.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.7 KB | Display | ![]() |
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Full document | ![]() | 460 KB | Display | |
Data in XML | ![]() | 12 KB | Display | |
Data in CIF | ![]() | 17.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1dulS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: RNA chain | Mass: 15930.493 Da / Num. of mol.: 1 / Fragment: RESIDUES 32-74 / Source method: obtained synthetically | ||||
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#2: Protein | Mass: 12042.420 Da / Num. of mol.: 1 / Fragment: C TERMINAL DOMAIN (RESIDUES 328-432) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
#3: Chemical | ChemComp-MG / #4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.12 Å3/Da / Density % sol: 60.62 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: evaporation / pH: 5.6 Details: Na-MES, isopropanol, potassium chloride, magnesium chloride, MPD, pH 5.6, EVAPORATION, temperature 293K | ||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Detector: CCD / Date: May 20, 1999 | ||||||||||||
Radiation | Monochromator: double crystal / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.52→40 Å / Num. all: 1178959 / Num. obs: 51967 / % possible obs: 98.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.8 % / Biso Wilson estimate: 19.8 Å2 / Rmerge(I) obs: 0.07 / Rsym value: 0.069 / Net I/σ(I): 35.3 | ||||||||||||
Reflection shell | Resolution: 1.52→1.57 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.49 / Mean I/σ(I) obs: 1.6 / Num. unique all: 4750 / % possible all: 89.3 | ||||||||||||
Reflection shell | *PLUS % possible obs: 89.3 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1DUL Resolution: 1.52→10 Å / Num. parameters: 1758 / Num. restraintsaints: 2208 / Cross valid method: FREE R / σ(F): 0 / σ(I): 0 / Stereochemistry target values: ENGH AND HUBER
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Refine analyze | Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 1952 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.52→10 Å
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Refine LS restraints |
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Software | *PLUS Name: SHELXL-97 / Classification: refinement | |||||||||||||||||||||||||||||||||
Refinement | *PLUS Num. reflection obs: 38752 | |||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |