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Open data
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Basic information
Entry | Database: PDB / ID: 1hfx | ||||||
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Title | ALPHA-LACTALBUMIN | ||||||
![]() | ALPHA-LACTALBUMIN | ||||||
![]() | GLYCOPROTEIN / LACTOSE SYNTHASE COMPONENT / CALCIUM BINDING METALLOPROTEIN / LACTOSE | ||||||
Function / homology | ![]() lactose synthase activity / lactose biosynthetic process / calcium ion binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Pike, A.C.W. / Brew, K. / Acharya, K.R. | ||||||
![]() | ![]() Title: Crystal structures of guinea-pig, goat and bovine alpha-lactalbumin highlight the enhanced conformational flexibility of regions that are significant for its action in lactose synthase. Authors: Pike, A.C. / Brew, K. / Acharya, K.R. #1: ![]() Title: Crystal Structure of Human Alpha-Lactalbumin at 1.7 A Resolution Authors: Acharya, K.R. / Ren, J.S. / Stuart, D.I. / Phillips, D.C. / Fenna, R.E. #2: ![]() Title: Refined Structure of Baboon Alpha-Lactalbumin at 1.7 A Resolution. Comparison with C-Type Lysozyme Authors: Acharya, K.R. / Stuart, D.I. / Walker, N.P. / Lewis, M. / Phillips, D.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 38.3 KB | Display | ![]() |
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PDB format | ![]() | 25.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 363.1 KB | Display | ![]() |
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Full document | ![]() | 363.2 KB | Display | |
Data in XML | ![]() | 3.7 KB | Display | |
Data in CIF | ![]() | 5.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14240.159 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#2: Chemical | ChemComp-CA / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.82 Å3/Da / Density % sol: 32 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / pH: 4.6 Details: 0.2M AMMONIUM SULFATE, 20-25% (W/V) PEG 4000, 0.1M SODIUM ACETATE PH4.6, 20MG/ML PROTEIN 20 DEGREES C, temperature 293K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: SIEMENS / Date: Feb 1, 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→39.1 Å / Num. obs: 8003 / % possible obs: 88 % / Observed criterion σ(I): 0 / Redundancy: 6 % / Rmerge(I) obs: 0.083 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: HUMAN ALPHA-LACTALBUMIN Resolution: 1.9→8 Å / σ(F): 0 /
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Displacement parameters | Biso mean: 20.08 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati d res low obs: 5 Å / Luzzati sigma a obs: 0.19 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→8 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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