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Open data
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Basic information
Entry | Database: PDB / ID: 1hfz | ||||||
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Title | ALPHA-LACTALBUMIN | ||||||
![]() | ALPHA-LACTALBUMIN | ||||||
![]() | GLYCOPROTEIN / LACTOSE SYNTHASE COMPONENT / CALCIUM BINDING METALLOPROTEIN / LACTOSE | ||||||
Function / homology | ![]() response to dehydroepiandrosterone / response to 11-deoxycorticosterone / lactose synthase activity / lactose biosynthetic process / response to progesterone / response to estradiol / lysozyme activity / calcium ion binding / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Pike, A.C.W. / Brew, K. / Acharya, K.R. | ||||||
![]() | ![]() Title: Crystal structures of guinea-pig, goat and bovine alpha-lactalbumin highlight the enhanced conformational flexibility of regions that are significant for its action in lactose synthase. Authors: Pike, A.C. / Brew, K. / Acharya, K.R. #1: ![]() Title: Study by Mutagenesis of the Roles of Two Aromatic Clusters of Alpha-Lactalbumin in Aspects of its Action in the Lactose Synthase System Authors: Grobler, J.A. / Wang, M. / Pike, A.C. / Brew, K. #2: ![]() Title: Crystal Structure of Human Alpha-Lactalbumin at 1.7 A Resolution Authors: Acharya, K.R. / Ren, J.S. / Stuart, D.I. / Phillips, D.C. / Fenna, R.E. #3: ![]() Title: Refined Structure of Baboon Alpha-Lactalbumin at 1.7 A Resolution. Comparison with C-Type Lysozyme Authors: Acharya, K.R. / Stuart, D.I. / Walker, N.P. / Lewis, M. / Phillips, D.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 107 KB | Display | ![]() |
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PDB format | ![]() | 88.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Details | THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT CONTAINS TWO DIMERS (CHAINS A AND B AND CHAINS C AND D). THE ADDITIONAL N-TERMINAL METHIONINE OF EACH CHAIN, INTRODUCED BY THE CLONING PROCEDURE, HAS BEEN NUMBERED 1X SO THAT THE REMAINING RESIDUES (1 - 123) HAVE A NUMBERING SCHEME THAT IS IDENTICAL TO OTHER ALPHA-LACTALBUMIN STRUCTURES. |
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Components
#1: Protein | Mass: 14301.180 Da / Num. of mol.: 4 / Mutation: INS(MET 1X), M90V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 8 / Details: pH 8.0 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 16 ℃ / Method: vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 24, 1994 | |||||||||
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Num. obs: 18629 / % possible obs: 80 % / Observed criterion σ(I): 0 / Redundancy: 2.8 % / Rmerge(I) obs: 0.078 | |||||||||
Reflection | *PLUS Highest resolution: 2.3 Å |
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Processing
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Refinement | Resolution: 2.3→8 Å / σ(F): 0 Details: CHAINS C AND D ARE GENERALLY POORLY DEFINED IN THE ELECTRON DENSITY DUE TO PACKING DISORDER WITHIN THE CRYSTAL. THIS IS REFLECTED IN THE RELATIVELY HIGH OVERALL TEMPERATURE FACTOR OBSERVED FOR THESE CHAINS.
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Displacement parameters | Biso mean: 48.39 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |