+Open data
-Basic information
Entry | Database: PDB / ID: 1ha4 | ||||||
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Title | GammaS crystallin C terminal domain from Homo Sapiens | ||||||
Components | GAMMA CRYSTALLIN S | ||||||
Keywords | EYE LENS PROTEIN / GAMMAS CRYSTALLIN | ||||||
Function / homology | Function and homology information structural constituent of eye lens / lens development in camera-type eye / visual perception / morphogenesis of an epithelium Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Purkiss, A.G. / Slingsby, C. / Bateman, O.A. / Goodfellow, J.M. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2002 Title: The X-Ray Crystal Structure of Human Gamma S-Crystallin C-Terminal Domain Authors: Purkiss, A.G. / Bateman, O.A. / Goodfellow, J.M. / Lubsen, N.H. / Slingsby, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ha4.cif.gz | 49.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ha4.ent.gz | 35.8 KB | Display | PDB format |
PDBx/mmJSON format | 1ha4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ha/1ha4 ftp://data.pdbj.org/pub/pdb/validation_reports/ha/1ha4 | HTTPS FTP |
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-Related structure data
Related structure data | 1a7hS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10427.862 Da / Num. of mol.: 2 / Fragment: C TERMINAL DOMAIN RESIDUES 91-177 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Cellular location: CYTOPLASM / Organ: EYE / Organelle: EYE-LENS / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P22914 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 6.308 Å3/Da / Density % sol: 61 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 5 Details: 0.1M SODIUM ACETATE, 0.2M AMMONIUM SULPHATE, 24% PEG MONOMETHYLETHER, pH 5.00 | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.6 / Wavelength: 1.5418 |
Detector | Date: Mar 18, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→48.7 Å / Num. obs: 10955 / % possible obs: 97.2 % / Redundancy: 6.5 % / Biso Wilson estimate: 32.439 Å2 / Rsym value: 0.057 / Net I/σ(I): 23.8 |
Reflection shell | Resolution: 2.4→2.46 Å / Redundancy: 5.9 % / Mean I/σ(I) obs: 5.7 / Rsym value: 0.107 / % possible all: 99.4 |
Reflection | *PLUS Num. measured all: 71559 / Rmerge(I) obs: 0.057 |
Reflection shell | *PLUS % possible obs: 99.4 % / Num. unique obs: 809 / Num. measured obs: 4790 / Rmerge(I) obs: 0.107 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1A7H Resolution: 2.4→100 Å / Data cutoff high absF: 10000 / Cross valid method: THROUGHOUT / σ(F): 0 Details: ALSO USED CCP4 PROGRAMS REFMAC, FFT, MAPMASK AND ARPP FOR SOME REFINEMENT ROUNDS
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Solvent computation | Solvent model: SHELL MODEL / Bsol: 49.795 Å2 / ksol: 0.409 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.904 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→100 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.46 Å / Total num. of bins used: 20
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 100 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |