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Open data
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Basic information
Entry | Database: PDB / ID: 1h97 | ||||||
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Title | Trematode hemoglobin from Paramphistomum epiclitum | ||||||
![]() | Globin-3 | ||||||
![]() | OXYGEN TRANSPORT | ||||||
Function / homology | ![]() oxygen carrier activity / oxygen binding / iron ion binding / heme binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pesce, A. / Dewilde, S. / Kiger, L. / Milani, M. / Ascenzi, P. / Marden, M.C. / Van Hauwaert, M.L. / Vanfleteren, J. / Moens, L. / Bolognesi, M. | ||||||
![]() | ![]() Title: Very High Resolution Structure of a Trematode Hemoglobin Displaying a Tyrb10-Tyre7 Heme Distal Residue Pair and High Oxygen Affinity Authors: Pesce, A. / Dewilde, S. / Kiger, L. / Milani, M. / Ascenzi, P. / Marden, M.C. / Van, M.L. / Vanfleteren, J. / Moens, L. / Bolognesi, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 147 KB | Display | ![]() |
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PDB format | ![]() | 116.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.9969, 0.06148, -0.0491), Vector: |
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Components
#1: Protein | Mass: 16641.053 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Cellular location: CYTOPLASM / Production host: ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 48 % | |||||||||||||||||||||||||
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Crystal grow | pH: 4.5 / Details: AMMONIUM SULFATE, PH 4.5 | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 15, 1999 / Details: MIRRORS | |||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.17→20 Å / Num. obs: 106124 / % possible obs: 91.5 % / Observed criterion σ(I): 0 / Redundancy: 4 % / Rmerge(I) obs: 0.051 / Rsym value: 0.051 / Net I/σ(I): 17 | |||||||||
Reflection shell | Resolution: 1.17→1.19 Å / Redundancy: 2 % / Rmerge(I) obs: 0.343 / Mean I/σ(I) obs: 3 / Rsym value: 0.343 / % possible all: 84 | |||||||||
Reflection shell | *PLUS % possible obs: 84.2 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Refine analyze | Num. disordered residues: 12 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.17→20 Å
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Refine LS restraints |
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Software | *PLUS Name: SHELXL-97 / Classification: refinement | |||||||||||||||||||||||||||||||||
Refinement | *PLUS % reflection Rfree: 10 % / Rfactor Rwork: 0.121 | |||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |