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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1h6k | ||||||
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タイトル | nuclear Cap Binding Complex | ||||||
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![]() | NUCLEAR PROTEIN / M7G CAP / CAP-BINDING-COMPLEX / RNP DOMAIN / MIF4G DOMAIN / RNA MATURATION / RNA EXPORT | ||||||
機能・相同性 | ![]() positive regulation of RNA binding / snRNA export from nucleus / nuclear cap binding complex / histone mRNA metabolic process / RNA cap binding complex / mRNA metabolic process / positive regulation of RNA export from nucleus / positive regulation of mRNA 3'-end processing / cap-dependent translational initiation / Processing of Intronless Pre-mRNAs ...positive regulation of RNA binding / snRNA export from nucleus / nuclear cap binding complex / histone mRNA metabolic process / RNA cap binding complex / mRNA metabolic process / positive regulation of RNA export from nucleus / positive regulation of mRNA 3'-end processing / cap-dependent translational initiation / Processing of Intronless Pre-mRNAs / snRNA binding / RNA cap binding / alternative mRNA splicing, via spliceosome / miRNA-mediated post-transcriptional gene silencing / primary miRNA processing / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / regulation of mRNA processing / regulatory ncRNA-mediated post-transcriptional gene silencing / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / RNA 7-methylguanosine cap binding / mRNA 3'-end processing / Transport of Mature mRNA Derived from an Intronless Transcript / positive regulation of mRNA splicing, via spliceosome / mRNA 3'-end processing / mRNA cis splicing, via spliceosome / Transport of Mature mRNA derived from an Intron-Containing Transcript / RNA catabolic process / regulation of translational initiation / RNA Polymerase II Transcription Termination / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / Abortive elongation of HIV-1 transcript in the absence of Tat / FGFR2 alternative splicing / Signaling by FGFR2 IIIa TM / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / spliceosomal complex assembly / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA polymerase II transcribes snRNA genes / 7-methylguanosine mRNA capping / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Formation of HIV-1 elongation complex containing HIV-1 Tat / mRNA export from nucleus / Formation of HIV elongation complex in the absence of HIV Tat / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of RNA Pol II elongation complex / RNA Polymerase II Pre-transcription Events / mRNA Splicing - Major Pathway / RNA splicing / positive regulation of transcription elongation by RNA polymerase II / mRNA transcription by RNA polymerase II / mRNA splicing, via spliceosome / Regulation of expression of SLITs and ROBOs / snRNP Assembly / positive regulation of cell growth / defense response to virus / molecular adaptor activity / ciliary basal body / ribonucleoprotein complex / mRNA binding / mitochondrion / DNA binding / RNA binding / nucleoplasm / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
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手法 | ![]() ![]() ![]() | ||||||
![]() | Mazza, C. / Ohno, M. / Segref, A. / Mattaj, I.W. / Cusack, S. | ||||||
![]() | ![]() タイトル: Crystal Structure of the Human Nuclear CAP Binding Complex 著者: Mazza, C. / Ohno, M. / Segref, A. / Mattaj, I.W. / Cusack, S. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 519.6 KB | 表示 | ![]() |
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PDB形式 | ![]() | 426.6 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 478.9 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 535.4 KB | 表示 | |
XML形式データ | ![]() | 104.2 KB | 表示 | |
CIF形式データ | ![]() | 150 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper:
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要素
#1: タンパク質 | 分子量: 87942.930 Da / 分子数: 3 / 断片: MIF4G DOMAIN / 変異: YES / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() #2: タンパク質 | 分子量: 11501.983 Da / 分子数: 3 / 断片: RNP DOMAIN / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #3: 水 | ChemComp-HOH / | 構成要素の詳細 | CHAIN A, B, C ENGINEERED MUTATION ALA479SER BINDS TO 5'CAPPED MRNA. AND INVOLVED IN MEDIATING U ...CHAIN A, B, C ENGINEERED | 配列の詳細 | RESIDUES 1-19 AND 671-684 FROM CBP80 (CHAINS A,B,C) HAVE BEEN REMOVED BY TRYPSIN RESIDUES 1-21, 78 ...RESIDUES 1-19 AND 671-684 FROM CBP80 (CHAINS A,B,C) HAVE BEEN REMOVED BY TRYPSIN RESIDUES 1-21, 78 AND 121-156 FROM CBP20 (CHAINS X,Y,Z) HAVE BEEN REMOVED BY TRYPSIN | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.1 Å3/Da / 溶媒含有率: 59.8 % | |||||||||||||||
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結晶化 | pH: 7.5 / 詳細: SEE REFERENCES, pH 7.50 | |||||||||||||||
結晶化 | *PLUS 温度: 4 ℃ / 手法: 蒸気拡散法, ハンギングドロップ法 | |||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC QUANTUM 4 / 検出器: CCD / 日付: 2000年2月15日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.933 Å / 相対比: 1 |
反射 | 解像度: 2→20 Å / Num. obs: 208094 / % possible obs: 83.1 % / 冗長度: 3.5 % / Rmerge(I) obs: 0.058 |
反射 シェル | 解像度: 2→2.03 Å / 冗長度: 3.5 % / Rmerge(I) obs: 0.363 / % possible all: 40.7 |
反射 | *PLUS 最低解像度: 20 Å / Num. measured all: 730893 |
反射 シェル | *PLUS % possible obs: 40.7 % |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]()
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2→20 Å
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拘束条件 |
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