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Open data
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Basic information
Entry | Database: PDB / ID: 1h3l | ||||||
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Title | N-terminal fragment of SigR from Streptomyces coelicolor | ||||||
![]() | RNA POLYMERASE SIGMA FACTOR | ||||||
![]() | TRANSCRIPTION / DNA-BINDING / TRANSCRIPTION REGULATION | ||||||
Function / homology | ![]() sigma factor activity / DNA-templated transcription initiation / response to oxidative stress / DNA-binding transcription factor activity / regulation of DNA-templated transcription / DNA binding Similarity search - Function | ||||||
Biological species | STREPTOMYCES COELICOLOR A3 | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Li, W. / Stevenson, C.E.M. / Burton, N. / Jakimowicz, P. / Paget, M.S.B. / Buttner, M.J. / Lawson, D.M. / Kleanthous, C. | ||||||
![]() | ![]() Title: Identification and Structure of the Anti-Sigma Factor-Binding Domain of the Disulfide-Stress Regulated Sigma Factor Sigma(R) from Streptomyces Coelicolor Authors: Li, W. / Stevenson, C.E.M. / Burton, N. / Jakimowicz, P. / Paget, M.S.B. / Buttner, M.J. / Lawson, D.M. / Kleanthous, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 42.1 KB | Display | ![]() |
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PDB format | ![]() | 30.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436.8 KB | Display | ![]() |
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Full document | ![]() | 442.6 KB | Display | |
Data in XML | ![]() | 8.6 KB | Display | |
Data in CIF | ![]() | 10.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.23955, -0.43961, -0.86566), Vector: |
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Components
#1: Protein | Mass: 10157.266 Da / Num. of mol.: 2 / Fragment: N-TERMINAL DOMAIN, RESIDUES 23-109 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: M145 / Plasmid: PET15B-SIGR / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | Sequence details | FRAGMENT PRODUCED BY PROTEOLYSI | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.69 Å3/Da / Density % sol: 66 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 Details: 10-15%(W/V) PEG 8000 IN 100MM TRIS-HCL PH8.5, pH 8.50 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Sep 15, 1999 |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.782 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→40 Å / Num. obs: 12713 / % possible obs: 99.6 % / Observed criterion σ(I): -3 / Redundancy: 3.8 % / Biso Wilson estimate: 43.8 Å2 / Rmerge(I) obs: 0.079 / Net I/σ(I): 25.2 |
Reflection shell | Resolution: 2.4→2.49 Å / Rmerge(I) obs: 0.302 / Mean I/σ(I) obs: 5.3 / % possible all: 99.9 |
Reflection | *PLUS % possible obs: 97.9 % / Rmerge(I) obs: 0.047 |
Reflection shell | *PLUS % possible obs: 83.4 % / Rmerge(I) obs: 0.142 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 34.368 Å2
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Refinement step | Cycle: LAST / Resolution: 2.375→40 Å
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Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 40 Å / % reflection Rfree: 5 % / Rfactor Rfree: 0.278 / Rfactor Rwork: 0.258 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2.375 Å / Lowest resolution: 2.436 Å / Rfactor Rfree: 0.346 / Rfactor Rwork: 0.287 / Num. reflection Rwork: 842 / Total num. of bins used: 20 |