PDB-2xvt: Structure of the extracellular domain of human RAMP2

ID or keywords:

Entry

Database: PDB / ID: 2xvt

Title

Structure of the extracellular domain of human RAMP2

Components

RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

Keywords

MEMBRANE PROTEIN /

TRANSMEMBRANE / RECEPTOR TRANSPORT / PROTEIN-TRAFFICKING /

GPCR / CRLR /

CGRP / ADRENOMEDULIN

Function / homology

Function and homology information

basement membrane assembly / vascular associated smooth muscle cell development /

adrenomedullin binding /

adrenomedullin receptor activity /

adrenomedullin receptor complex / adrenomedullin receptor signaling pathway / Calcitonin-like ligand receptors / positive regulation of vasculogenesis / bicellular tight junction assembly / regulation of G protein-coupled receptor signaling pathway ...
Similarity search - Function

Biological species

HOMO SAPIENS (human)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MAD / Resolution: 2.05 Å

Authors

Quigley, A. / Pike, A.C.W. / Burgess-Brown, N. / Krojer, T. / Shrestha, L. / Goubin, S. / Kim, J. / Das, S. / Muniz, J.R.C. / Canning, P. ...

Citation

Journal: To be Published
Title: Structure of the Extracellular Domain of Human Ramp2
Authors: Quigley, A. / Pike, A.C.W. / Burgess-Brown, N. / Krojer, T. / Shrestha, L. / Goubin, S. / Kim, J. / Das, S. / Muniz, J.R.C. / Canning, P. / Chaikuad, A. / Vollmar, M. / von Delft, F. / ...

History

Deposition	Oct 31, 2010	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Dec 29, 2010	Provider: repository / Type: Initial release
Revision 1.1	Mar 6, 2013	Group: Data collection
Revision 1.2	Dec 10, 2014	Group: Data collection / Version format compliance
Revision 1.3	Jan 24, 2018	Group: Database references / Category: citation_author / Item: _citation_author.name

Structure viewer	Molecule: MolmilJmol/JSmol

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PDBx/mmCIF format	2xvt.cif.gz	209.5 KB	Display	PDBx/mmCIF format
PDB format	pdb2xvt.ent.gz	179 KB	Display	PDB format
PDBx/mmJSON format	2xvt.json.gz		Tree view	PDBx/mmJSON format
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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/xv/2xvt ftp://data.pdbj.org/pub/pdb/validation_reports/xv/2xvt	HTTPS FTP

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Related structure data

Similar structure data	3aqe-8 4hzm-2 1iu1-d 3aqe-7 1tl2-d 4a9m-1 3ay8-d 4od5-6 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

Similar structure data

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

B: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

C: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

D: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

E: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

F: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

hetero molecules

65.4 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

D: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

E: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

F: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

hetero molecules

defined by software
32.7 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

A: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

B: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

C: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

hetero molecules

defined by software
32.7 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

A: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

defined by author
10.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

B: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

defined by author
10.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

5

C: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

hetero molecules

defined by author
10.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

6

D: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

defined by author
10.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

7

E: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

defined by author
10.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

8

F: RECEPTOR ACTIVITY-MODIFYING PROTEIN 2

hetero molecules

defined by author
11 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	59.759, 90.984, 92.056
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	19
Space group name H-M	P2₁2₁2₁

Noncrystallographic symmetry (NCS)

NCS domain:
NCS domain segments:

Dom-ID	Component-ID	Ens-ID	Refine code	Auth asym-ID	Auth seq-ID
1	1	1	2	A	61 - 69
2	1	1	2	B	61 - 69
3	1	1	2	C	61 - 69
4	1	1	2	D	61 - 69
5	1	1	2	E	61 - 69
6	1	1	2	F	61 - 69
1	1	2	5	A	70 - 71
2	1	2	5	B	70 - 71
3	1	2	5	C	70 - 71
4	1	2	5	D	70 - 71
5	1	2	5	E	70 - 71
6	1	2	5	F	70 - 71
1	1	3	2	A	72 - 76
2	1	3	2	B	72 - 76
3	1	3	2	C	72 - 76
4	1	3	2	D	72 - 76
5	1	3	2	E	72 - 76
6	1	3	2	F	72 - 76
1	1	4	6	A	77 - 80
2	1	4	6	B	77 - 80
3	1	4	6	C	77 - 80
4	1	4	6	D	77 - 80
5	1	4	6	E	77 - 80
6	1	4	6	F	77 - 80
1	1	5	2	A	81 - 87
2	1	5	2	B	81 - 87
3	1	5	2	C	81 - 87
4	1	5	2	D	81 - 87
5	1	5	2	E	81 - 87
6	1	5	2	F	81 - 87
1	1	6	4	A	105 - 110
2	1	6	4	B	105 - 110
3	1	6	4	C	105 - 110
4	1	6	4	D	105 - 110
5	1	6	4	E	105 - 110
6	1	6	4	F	105 - 110
1	1	7	2	A	111 - 117
2	1	7	2	B	111 - 117
3	1	7	2	C	111 - 117
4	1	7	2	D	111 - 117
5	1	7	2	E	111 - 117
6	1	7	2	F	111 - 117
1	1	8	3	A	119 - 128
2	1	8	3	B	119 - 128
3	1	8	3	C	119 - 128
4	1	8	3	D	119 - 128
5	1	8	3	E	119 - 128
6	1	8	3	F	119 - 128
1	1	9	6	A	129 - 132
2	1	9	6	B	129 - 132
3	1	9	6	C	129 - 132
4	1	9	6	D	129 - 132
5	1	9	6	E	129 - 132
6	1	9	6	F	129 - 132
1	1	10	3	A	88 - 95
2	1	10	3	B	88 - 95
3	1	10	3	C	88 - 95
4	1	10	3	D	88 - 95
5	1	10	3	E	88 - 95
6	1	10	3	F	88 - 95
1	1	11	3	A	96 - 104
2	1	11	3	B	96 - 104
3	1	11	3	C	96 - 104
4	1	11	3	D	96 - 104
5	1	11	3	E	96 - 104
6	1	11	3	F	96 - 104

NCS ensembles :

ID
1
2
3
4
5
6
7
8
9
10
11

NCS oper:

ID	Code	Matrix	Vector
1	given	(-0.47561, 0.73733, -0.47972), (-0.85187, -0.25008, 0.4602), (0.21935, 0.62753, 0.74705)	76.90105, 73.44485, -24.33546
2	given	(-0.492, -0.852, 0.177), (0.715, -0.28, 0.641), (-0.497, 0.442, 0.747)	106.11459, -21.31891, 22.70615
3	given	(0.482, -0.732, 0.481), (-0.85, -0.257, 0.46), (-0.213, -0.631, -0.746)	16.38894, 73.23527, 70.09725
4	given	(-1, 0.008, 0.018), (0.008, 1, -0.01), (-0.019, -0.01, -1)	93.41971, -0.67687, 47.44164
5	given	(0.485, 0.856, -0.179), (0.732, -0.285, 0.619), (0.479, -0.432, -0.764)	-11.76748, -22.21119, 24.33679

#1: Protein	RECEPTOR ACTIVITY-MODIFYING PROTEIN 2 / CALCITONIN-RECEPTOR-LIKE RECEPTOR ACTIVITY-MODIFYING PROTEIN 2 / CRLR ACTIVITY-MODIFYING PROTEIN 2 / RAMP2 Mass: 10873.156 Da / Num. of mol.: 6 / Fragment: EXTRACELLULAR DOMAIN, RESIDUES 48-139 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PNIC28-BSA4 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): R3-PRARE2 / References: UniProt: O60895
#2: Chemical	ChemComp-CA / CALCIUM ION Mass: 40.078 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Ca
#3: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 228 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 2

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Sample preparation

Crystal	Density Matthews: 1.75 Å³/Da / Density % sol: 35.87 % / Description: NONE
Crystal grow	pH: 7.5 Details: 0.1M CALCIUM CHLORIDE, 26% PEG MONOMETHYLETHER 350, 0.1M HEPES PH 7.5

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Data collection

Diffraction

ID	Mean temperature (K)	Crystal-ID
1	100	1
2	100	1

Diffraction source

Source	Site	Beamline	ID	Wavelength	Wavelength (Å)
SYNCHROTRON	Diamond	I02	1	0.9795
SYNCHROTRON	Diamond	I04	2		0.9803, 0.9806, 0.9763

Detector

Type	ID	Detector	Date	Details
ADSC CCD	1	CCD	Aug 11, 2010	NULL: NULL
ADSC CCD	2	CCD

Radiation

ID	Protocol	Monochromatic (M) / Laue (L)	Scattering type	Wavelength-ID
1	SINGLE WAVELENGTH	M	x-ray	1
2	MAD	M	x-ray	1

Radiation wavelength

ID	Wavelength (Å)	Relative weight
1	0.9795	1
2	0.9803	1
3	0.9806	1
4	0.9763	1

Reflection

Resolution: 2.05→50.13 Å / Num. obs: 32252 / % possible obs: 99.7 % / Observed criterion σ(I): 0 / Redundancy: 6.7 % / B_iso Wilson estimate: 22.244 Å² / R_merge(I) obs: 0.16 / Net I/σ(I): 7.8

Reflection shell

Resolution: 2.05→2.16 Å / Redundancy: 4.6 % / R_merge(I) obs: 0.77 / Mean I/σ(I) obs: 2 / % possible all: 98.2

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Processing

Software

Name	Version	Classification
REFMAC	5.5.0110	refinement
MOSFLM		data reduction
SCALA		data scaling
autoSHARP		phasing

Refinement

Method to determine structure:

MAD
Starting model: NONE

Resolution: 2.05→64.71 Å / Cor.coef. F_o:F_c: 0.958 / Cor.coef. F_o:F_c free: 0.934 / SU B: 10.876 / SU ML: 0.143 / Cross valid method: THROUGHOUT / ESU R: 0.226 / ESU R Free: 0.19 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.24636	1627	5.1 %	RANDOM
R_work	0.1969	-	-	-
obs	0.19944	30525	99.74 %	-

Solvent computation

Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å

Displacement parameters

B_iso mean: 21.316 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	-1.74 Å²	0 Å²	0 Å²
2-	-	2.04 Å²	0 Å²
3-	-	-	-0.3 Å²

Refinement step

Cycle: LAST / Resolution: 2.05→64.71 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	3924	0	3	228	4155

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.016	0.021	4092
X-RAY DIFFRACTION	r_bond_other_d	0.001	0.02	2724
X-RAY DIFFRACTION	r_angle_refined_deg	1.559	1.906	5579
X-RAY DIFFRACTION	r_angle_other_deg	1.477	3.007	6551
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	6.21	5	463
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	35.658	24.103	234
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	14.007	15	629
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	14.59	15	21
X-RAY DIFFRACTION	r_chiral_restr	0.105	0.2	564
X-RAY DIFFRACTION	r_gen_planes_refined	0.008	0.021	4560
X-RAY DIFFRACTION	r_gen_planes_other	0.001	0.02	911
X-RAY DIFFRACTION	r_nbd_refined	0.224	0.2	859
X-RAY DIFFRACTION	r_nbd_other	0.17	0.2	2640
X-RAY DIFFRACTION	r_nbtor_refined	0.193	0.2	1986
X-RAY DIFFRACTION	r_nbtor_other	0.09	0.2	1840
X-RAY DIFFRACTION	r_xyhbond_nbd_refined	0.187	0.2	199
X-RAY DIFFRACTION	r_xyhbond_nbd_other	0.022	0.2	1
X-RAY DIFFRACTION	r_metal_ion_refined	0.162	0.2	12
X-RAY DIFFRACTION	r_metal_ion_other
X-RAY DIFFRACTION	r_symmetry_vdw_refined	0.2	0.2	24
X-RAY DIFFRACTION	r_symmetry_vdw_other	0.183	0.2	55
X-RAY DIFFRACTION	r_symmetry_hbond_refined	0.145	0.2	18
X-RAY DIFFRACTION	r_symmetry_hbond_other
X-RAY DIFFRACTION	r_symmetry_metal_ion_refined
X-RAY DIFFRACTION	r_symmetry_metal_ion_other
X-RAY DIFFRACTION	r_mcbond_it	2.365	3	2359
X-RAY DIFFRACTION	r_mcbond_other	0.844	3	912
X-RAY DIFFRACTION	r_mcangle_it	3.332	5	3831
X-RAY DIFFRACTION	r_mcangle_other
X-RAY DIFFRACTION	r_scbond_it	5.463	8	1733
X-RAY DIFFRACTION	r_scbond_other
X-RAY DIFFRACTION	r_scangle_it	6.767	11	1748
X-RAY DIFFRACTION	r_scangle_other
X-RAY DIFFRACTION	r_long_range_B_refined
X-RAY DIFFRACTION	r_long_range_B_other
X-RAY DIFFRACTION	r_rigid_bond_restr
X-RAY DIFFRACTION	r_sphericity_free
X-RAY DIFFRACTION	r_sphericity_bonded

Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Weight position: 10

Ens-ID	Dom-ID	Auth asym-ID	Number	Type	Rms dev position (Å)
1	1	A	54	tight positional	0.09
1	2	B	54	tight positional	0.09
1	3	C	54	tight positional	0.1
1	4	D	54	tight positional	0.09
1	5	E	54	tight positional	0.08
1	6	F	54	tight positional	0.08
3	1	A	30	tight positional	0.16
3	2	B	30	tight positional	0.07
3	3	C	30	tight positional	0.09
3	4	D	30	tight positional	0.11
3	5	E	30	tight positional	0.09
3	6	F	30	tight positional	0.12
5	1	A	42	tight positional	0.09
5	2	B	42	tight positional	0.09
5	3	C	42	tight positional	0.13
5	4	D	42	tight positional	0.11
5	5	E	42	tight positional	0.12
5	6	F	42	tight positional	0.09
7	1	A	40	tight positional	0.09
7	2	B	40	tight positional	0.09
7	3	C	40	tight positional	0.09
7	4	D	40	tight positional	0.06
7	5	E	40	tight positional	0.07
7	6	F	40	tight positional	0.1
8	1	A	60	tight positional	0.09
8	2	B	60	tight positional	0.09
8	3	C	60	tight positional	0.13
8	4	D	60	tight positional	0.14
8	5	E	60	tight positional	0.1
8	6	F	60	tight positional	0.11
10	1	A	47	tight positional	0.11
10	2	B	47	tight positional	0.12
10	3	C	47	tight positional	0.14
10	4	D	47	tight positional	0.12
10	5	E	47	tight positional	0.12
10	6	F	47	tight positional	0.19
11	1	A	54	tight positional	0.08
11	2	B	54	tight positional	0.08
11	3	C	54	tight positional	0.08
11	4	D	54	tight positional	0.14
11	5	E	54	tight positional	0.09
11	6	F	54	tight positional	0.09
1	1	A	78	medium positional	0.19
1	2	B	78	medium positional	0.18
1	3	C	78	medium positional	0.21
1	4	D	78	medium positional	0.25
1	5	E	78	medium positional	0.18
1	6	F	78	medium positional	0.18
2	1	A	12	medium positional	0.54
2	2	B	12	medium positional	0.29
2	3	C	12	medium positional	0.31
2	4	D	12	medium positional	0.25
2	5	E	12	medium positional	0.58
2	6	F	12	medium positional	0.33
3	1	A	41	medium positional	0.28
3	2	B	41	medium positional	0.18
3	3	C	41	medium positional	0.17
3	4	D	41	medium positional	0.15
3	5	E	41	medium positional	0.19
3	6	F	41	medium positional	0.18
5	1	A	58	medium positional	0.22
5	2	B	58	medium positional	0.19
5	3	C	58	medium positional	0.23
5	4	D	58	medium positional	0.19
5	5	E	58	medium positional	0.2
5	6	F	58	medium positional	0.19
6	1	A	75	medium positional	0.26
6	2	B	75	medium positional	0.17
6	3	C	75	medium positional	0.18
6	4	D	75	medium positional	0.24
6	5	E	75	medium positional	0.22
6	6	F	75	medium positional	0.2
7	1	A	55	medium positional	0.14
7	2	B	55	medium positional	0.13
7	3	C	55	medium positional	0.15
7	4	D	55	medium positional	0.16
7	5	E	55	medium positional	0.15
7	6	F	55	medium positional	0.23
2	1	A	16	loose positional	1.31
2	2	B	16	loose positional	0.62
2	3	C	16	loose positional	0.63
2	4	D	16	loose positional	0.66
2	5	E	16	loose positional	1.22
2	6	F	16	loose positional	0.64
4	1	A	54	loose positional	0.26
4	2	B	54	loose positional	0.23
4	3	C	54	loose positional	0.46
4	4	D	54	loose positional	0.25
4	5	E	54	loose positional	0.27
4	6	F	54	loose positional	0.39
8	1	A	91	loose positional	0.38
8	2	B	91	loose positional	0.45
8	3	C	91	loose positional	0.56
8	4	D	91	loose positional	0.44
8	5	E	91	loose positional	0.42
8	6	F	91	loose positional	0.55
9	1	A	40	loose positional	0.5
9	2	B	40	loose positional	0.23
9	3	C	40	loose positional	0.25
9	4	D	40	loose positional	0.25
9	5	E	40	loose positional	0.55
9	6	F	40	loose positional	0.26
10	1	A	61	loose positional	0.63
10	2	B	61	loose positional	0.73
10	3	C	61	loose positional	0.64
10	4	D	61	loose positional	0.68
10	5	E	61	loose positional	0.6
10	6	F	61	loose positional	0.81
11	1	A	77	loose positional	0.14
11	2	B	77	loose positional	0.21
11	3	C	77	loose positional	0.17
11	4	D	77	loose positional	0.23
11	5	E	77	loose positional	0.15
11	6	F	77	loose positional	0.19
1	1	A	54	tight thermal	1.87
1	2	B	54	tight thermal	1.57
1	3	C	54	tight thermal	1.64
1	4	D	54	tight thermal	1.59
1	5	E	54	tight thermal	1.38
1	6	F	54	tight thermal	1.41
3	1	A	30	tight thermal	3.54
3	2	B	30	tight thermal	1.77
3	3	C	30	tight thermal	2.89
3	4	D	30	tight thermal	2.03
3	5	E	30	tight thermal	3.18
3	6	F	30	tight thermal	2.71
5	1	A	42	tight thermal	1.67
5	2	B	42	tight thermal	1.65
5	3	C	42	tight thermal	1.81
5	4	D	42	tight thermal	1.18
5	5	E	42	tight thermal	1.27
5	6	F	42	tight thermal	1.54
7	1	A	40	tight thermal	1.46
7	2	B	40	tight thermal	1.8
7	3	C	40	tight thermal	1.63
7	4	D	40	tight thermal	1.54
7	5	E	40	tight thermal	1.24
7	6	F	40	tight thermal	1.4
8	1	A	60	tight thermal	1.93
8	2	B	60	tight thermal	1.24
8	3	C	60	tight thermal	1.8
8	4	D	60	tight thermal	2.03
8	5	E	60	tight thermal	1.81
8	6	F	60	tight thermal	1.28
10	1	A	47	tight thermal	1.62
10	2	B	47	tight thermal	1.8
10	3	C	47	tight thermal	1.53
10	4	D	47	tight thermal	1.63
10	5	E	47	tight thermal	1.41
10	6	F	47	tight thermal	2.22
11	1	A	54	tight thermal	1.49
11	2	B	54	tight thermal	1.29
11	3	C	54	tight thermal	1.46
11	4	D	54	tight thermal	1.67
11	5	E	54	tight thermal	1.56
11	6	F	54	tight thermal	1.34
1	1	A	78	medium thermal	3.16
1	2	B	78	medium thermal	3.77
1	3	C	78	medium thermal	3.69
1	4	D	78	medium thermal	3.1
1	5	E	78	medium thermal	3.2
1	6	F	78	medium thermal	2.71
2	1	A	12	medium thermal	4
2	2	B	12	medium thermal	1.55
2	3	C	12	medium thermal	1.67
2	4	D	12	medium thermal	3.09
2	5	E	12	medium thermal	2.57
2	6	F	12	medium thermal	2.04
3	1	A	41	medium thermal	4.42
3	2	B	41	medium thermal	1.99
3	3	C	41	medium thermal	3.41
3	4	D	41	medium thermal	2.97
3	5	E	41	medium thermal	4.19
3	6	F	41	medium thermal	3.23
5	1	A	58	medium thermal	2.15
5	2	B	58	medium thermal	2.18
5	3	C	58	medium thermal	2.29
5	4	D	58	medium thermal	2.01
5	5	E	58	medium thermal	2.8
5	6	F	58	medium thermal	2.45
6	1	A	75	medium thermal	2.36
6	2	B	75	medium thermal	2.55
6	3	C	75	medium thermal	2.5
6	4	D	75	medium thermal	2.72
6	5	E	75	medium thermal	3.43
6	6	F	75	medium thermal	2.87
7	1	A	55	medium thermal	1.83
7	2	B	55	medium thermal	1.7
7	3	C	55	medium thermal	1.79
7	4	D	55	medium thermal	1.86
7	5	E	55	medium thermal	1.02
7	6	F	55	medium thermal	1.46
2	1	A	16	loose thermal	4.23
2	2	B	16	loose thermal	5.98
2	3	C	16	loose thermal	2.44
2	4	D	16	loose thermal	4.21
2	5	E	16	loose thermal	4.31
2	6	F	16	loose thermal	4.04
4	1	A	54	loose thermal	2.4
4	2	B	54	loose thermal	3.09
4	3	C	54	loose thermal	3.1
4	4	D	54	loose thermal	2.77
4	5	E	54	loose thermal	3.04
4	6	F	54	loose thermal	3.03
8	1	A	91	loose thermal	2.3
8	2	B	91	loose thermal	2.55
8	3	C	91	loose thermal	2.02
8	4	D	91	loose thermal	2.63
8	5	E	91	loose thermal	2.19
8	6	F	91	loose thermal	2.33
9	1	A	40	loose thermal	3.87
9	2	B	40	loose thermal	2.21
9	3	C	40	loose thermal	3.21
9	4	D	40	loose thermal	1.85
9	5	E	40	loose thermal	4.26
9	6	F	40	loose thermal	3.24
10	1	A	61	loose thermal	2.53
10	2	B	61	loose thermal	4.44
10	3	C	61	loose thermal	4.7
10	4	D	61	loose thermal	4.29
10	5	E	61	loose thermal	2.58
10	6	F	61	loose thermal	2.99
11	1	A	77	loose thermal	2.11
11	2	B	77	loose thermal	2.18
11	3	C	77	loose thermal	2.09
11	4	D	77	loose thermal	2.62
11	5	E	77	loose thermal	2.36
11	6	F	77	loose thermal	2.25

LS refinement shell

Resolution: 2.049→2.103 Å / Total num. of bins used: 20

	R_factor	Num. reflection	% reflection
R_free	0.339	127	-
R_work	0.322	2192	-
obs	-	-	98.72 %

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	3.2629	0.295	0.2789	10.7463	-1.648	3.7402	-0.0301	-0.1603	-0.2865	0.1087	0.0257	-0.7255	0.0072	0.2331	0.0044	0.004	-0.0113	-0.0113	0.1628	0.0214	0.104	72.1425	21.7973	13.6465
2	5.1786	-0.7365	2.5869	1.3971	-0.9578	5.9878	-0.0366	-0.0946	-0.2053	-0.0163	0.0385	-0.036	0.1627	-0.092	-0.0019	0.0703	-0.0441	0.0121	0.1027	0.031	0.0739	51.7847	13.0897	15.1465
3	5.2973	1.1941	-1.4856	2.8738	-0.8236	2.703	0.143	0.0009	0.3892	0.0447	0.0142	0.2455	-0.2321	-0.1224	-0.1572	0.0948	0.0236	-0.002	0.1027	0.0121	0.0475	55.0475	33.0384	6.5274
4	5.7244	0.1651	2.3636	1.396	0.3435	4.8104	-0.0588	0.2062	-0.2112	0.0011	0.0605	0.0738	0.1693	-0.0005	-0.0017	0.0847	0.0326	0.0201	0.0929	-0.018	0.0585	42.0061	12.5654	30.8212
5	3.5622	-1.0252	-0.1991	11.3243	2.2265	3.3861	-0.072	0.1178	-0.3269	-0.0657	0.0166	0.9268	-0.0102	-0.2454	0.0554	0.0054	0.0144	-0.0084	0.199	-0.0126	0.1301	21.6256	21.6706	32.1642
6	5.8905	-1.3334	-3.173	3.0934	0.9091	3.7745	0.1652	-0.0353	0.5002	-0.0101	0.0383	-0.3079	-0.2038	0.055	-0.2035	0.101	-0.0133	-0.0173	0.062	-0.0128	0.0865	38.7508	32.9936	39.2865

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	A	58 - 135
2	X-RAY DIFFRACTION	2	B	58 - 135
3	X-RAY DIFFRACTION	3	C	58 - 136
4	X-RAY DIFFRACTION	4	D	57 - 135
5	X-RAY DIFFRACTION	5	E	58 - 134
6	X-RAY DIFFRACTION	6	F	58 - 136

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