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Open data
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Basic information
Entry | Database: PDB / ID: 1gxq | ||||||
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Title | Crystal structure of the PhoB effector domain | ||||||
![]() | PHOSPHATE REGULON TRANSCRIPTIONAL REGULATORY PROTEIN PHOB | ||||||
![]() | TRANSCRIPTIONAL ACTIVATOR / HELIX-WINGED-HELIX / SENSORY TRANSDUCTION / PHOSPHORYLATION / DNA BINDING / ACTIVATOR / TWO-COMPONENT SIGNAL TRANSDUCTION | ||||||
Function / homology | ![]() bacterial-type RNA polymerase holo enzyme binding / phosphate ion transport / phosphorelay response regulator activity / regulation of DNA-templated transcription initiation / protein-DNA complex / transcription cis-regulatory region binding / regulation of DNA-templated transcription / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Blanco, A.G. / Sola, M. / Gomis-Ruth, F.X. / Coll, M. | ||||||
![]() | ![]() Title: Tandem DNA Recognition by Two-Component Signal Transduction Transcriptional Activator Phob Authors: Blanco, A.G. / Sola, M. / Gomis-Ruth, F.X. / Coll, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 35.3 KB | Display | ![]() |
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PDB format | ![]() | 23.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 358.6 KB | Display | ![]() |
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Full document | ![]() | 361 KB | Display | |
Data in XML | ![]() | 3.4 KB | Display | |
Data in CIF | ![]() | 5.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1gxpC ![]() 1qqiS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 12358.092 Da / Num. of mol.: 1 Fragment: DNA-BINDING AND TRANSACTIVATION DOMAIN, UNP RESIDUES 124-229 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 68 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.50 | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: Dec 15, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2→21.2 Å / Num. obs: 6550 / % possible obs: 94.4 % / Observed criterion σ(I): 2 / Redundancy: 9.5 % / Rmerge(I) obs: 0.072 / Net I/σ(I): 6.3 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.206 / Mean I/σ(I) obs: 3.1 / % possible all: 71.1 |
Reflection | *PLUS Num. measured all: 62124 |
Reflection shell | *PLUS % possible obs: 71.1 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1QQI Resolution: 2→21 Å / Cross valid method: THROUGHOUT / σ(F): 2 / Details: DISORDERED REGIONS WERE MODELED STEREOCHEMICALLY
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Displacement parameters | Biso mean: 25.54 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→21 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2 Å / Rfactor obs: 0.2 / Rfactor Rwork: 0.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.285 |