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Open data
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Basic information
Entry | Database: PDB / ID: 1gsh | ||||||
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Title | STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5 | ||||||
![]() | GLUTATHIONE BIOSYNTHETIC LIGASE | ||||||
![]() | GLUTATHIONE BIOSYNTHESIS LIGASE / GLUTATHIONE BIOSYNTHESIS / GLUTATHIONE SYNTHASE | ||||||
Function / homology | ![]() glutathione synthase / glutathione synthase activity / glutathione biosynthetic process / protein homotetramerization / magnesium ion binding / ATP binding / identical protein binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Matsuda, K. / Kato, H. / Yamaguchi, H. / Nishioka, T. / Katsube, Y. / Oda, J. | ||||||
![]() | ![]() Title: Crystal structure of glutathione synthetase at optimal pH: domain architecture and structural similarity with other proteins. Authors: Matsuda, K. / Mizuguchi, K. / Nishioka, T. / Kato, H. / Go, N. / Oda, J. #1: ![]() Title: Flexible Loop that is Novel Catalytic Machinery in a Ligase. Atomic Structure and Function of the Loopless Glutathione Synthetase Authors: Kato, H. / Tanaka, T. / Yamaguchi, H. / Hara, T. / Nishioka, T. / Katsube, Y. / Oda, J. #2: ![]() Title: Mechanism-Based Inactivation of Glutathione Synthetase by Phosphinic Acid Transition-State Analogue Authors: Hiratake, J. / Kato, H. / Oda, J. #3: ![]() Title: Use of Adenosine (5')Polyphospho(5')Pyridoxals to Study the Substrate-Binding Region of Glutathione Synthetase from Escherichia Coli B Authors: Hibi, T. / Kato, H. / Nishioka, T. / Oda, J. / Yamaguchi, H. / Katsube, Y. / Tanizawa, K. / Fukui, T. #4: ![]() Title: Flexibility Impaired by Mutations Revealed the Multifunctional Roles of the Loop in Glutathione Synthetase Authors: Tanaka, T. / Yamaguchi, H. / Kato, H. / Nishioka, T. / Katsube, Y. / Oda, J. #5: ![]() Title: Three-Dimensional Structure of the Glutathione Synthetase from Escherichia Coli B at 2.0 A Resolution Authors: Yamaguchi, H. / Kato, H. / Hata, Y. / Nishioka, T. / Kimura, A. / Oda, J. / Katsube, Y. #6: ![]() Title: Structural Studies on Glutathione Synthetase from Escherichia Coli B Authors: Yamaguchi, H. / Kato, H. / Hata, Y. / Nishioka, T. / Oda, J. / Katsube, Y. #7: ![]() Title: Crystallization and Preliminary X-Ray Studies of Glutathione Synthetase from Escherichia Coli B Authors: Kato, H. / Yamaguchi, H. / Hata, Y. / Nishioka, T. / Katsube, Y. / Oda, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 73.3 KB | Display | ![]() |
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PDB format | ![]() | 55.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 369.3 KB | Display | ![]() |
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Full document | ![]() | 376.2 KB | Display | |
Data in XML | ![]() | 7.8 KB | Display | |
Data in CIF | ![]() | 11.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35601.824 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.81 Å3/Da / Density % sol: 44 % | ||||||||||||||||||||||||
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Crystal grow | Method: microdialysis / pH: 7.5 Details: CRYSTALS WERE PREPARED AT PH 7.5 BY MICRODIALYSIS METHOD WITH AMMONIUM SULFATE AS THE PRECIPITATING AGENT. THE INNER SOLUTION 100 MICROLITER CONTAINED 1.5 % (W/V) GSHASE, 5 MM MGCL2 AND 10 % ...Details: CRYSTALS WERE PREPARED AT PH 7.5 BY MICRODIALYSIS METHOD WITH AMMONIUM SULFATE AS THE PRECIPITATING AGENT. THE INNER SOLUTION 100 MICROLITER CONTAINED 1.5 % (W/V) GSHASE, 5 MM MGCL2 AND 10 % SATURATED AMMONIUM SULFATE IN 50 MM TRIS-HCL BUFFER (PH 7.5). THE INNER SOLUTION WAS DIALYZED AGAINST 50 MM TRIS-HCL BUFFER (PH 7.5) WHICH CONTAINED 5 MM MGCL2 AND 25 % SATURATED AMMONIUM SULFATE.TRIS-HCL BUFFER, microdialysis | ||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Mar 18, 1991 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.04 Å / Relative weight: 1 |
Reflection | Highest resolution: 2 Å / Num. obs: 30967 / % possible obs: 90.3 % / Observed criterion σ(I): 1 / Rmerge(I) obs: 0.07 |
Reflection | *PLUS Highest resolution: 1.8 Å / % possible obs: 84.1 % / Rmerge(I) obs: 0.0701 |
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Processing
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Refinement | Resolution: 2→10 Å / σ(F): 2
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Displacement parameters | Biso mean: 18.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.18 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS % reflection Rfree: 10 % / Rfactor Rfree: 23.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_dihedral_angle_d / Dev ideal: 25.359 |