+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1gq5 | ||||||
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タイトル | Structural Determinants of the NHERF Interaction with beta2-AR and PDGFR | ||||||
要素 | EZRIN-RADIXIN-MOESIN BINDING PHOSPHOPROTEIN-50 | ||||||
キーワード | SIGNALING PROTEIN / PDZ / BETA2-ADRENERGIC RECEPTOR / PDGFR / NHERF / COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 platelet activating factor receptor activity / platelet-derived growth factor receptor activity / platelet-derived growth factor beta-receptor activity / cell migration involved in coronary angiogenesis / metanephric glomerular mesangial cell proliferation involved in metanephros development / positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway / smooth muscle cell chemotaxis / import across plasma membrane / channel activator activity / regulation of renal phosphate excretion ...platelet activating factor receptor activity / platelet-derived growth factor receptor activity / platelet-derived growth factor beta-receptor activity / cell migration involved in coronary angiogenesis / metanephric glomerular mesangial cell proliferation involved in metanephros development / positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway / smooth muscle cell chemotaxis / import across plasma membrane / channel activator activity / regulation of renal phosphate excretion / renal sodium ion transport / type 2 metabotropic glutamate receptor binding / glutathione transport / metanephric glomerular capillary formation / dopamine receptor binding / renal phosphate ion absorption / gamma-aminobutyric acid transmembrane transporter activity / gamma-aminobutyric acid import / cerebrospinal fluid circulation / cell migration involved in vasculogenesis / microvillus assembly / positive regulation of monoatomic ion transmembrane transport / aorta morphogenesis / negative regulation of sodium ion transport / bile acid secretion / positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway / platelet-derived growth factor binding / maintenance of epithelial cell apical/basal polarity / plasma membrane organization / stereocilium tip / cilium organization / intracellular phosphate ion homeostasis / vascular endothelial growth factor binding / retina vasculature development in camera-type eye / cardiac myofibril assembly / gland morphogenesis / myosin II binding / phosphatidylinositol metabolic process / phospholipase C-activating dopamine receptor signaling pathway / growth factor receptor binding / establishment of Golgi localization / fibroblast migration / positive regulation of chemotaxis / Signaling by PDGF / type 3 metabotropic glutamate receptor binding / plasma membrane protein complex / establishment of epithelial cell apical/basal polarity / negative regulation of fibroblast migration / platelet-derived growth factor receptor binding / chloride channel regulator activity / negative regulation of platelet-derived growth factor receptor signaling pathway / auditory receptor cell stereocilium organization / nuclear migration / regulation of protein kinase activity / beta-2 adrenergic receptor binding / microvillus membrane / regulation of cell size / positive regulation of DNA biosynthetic process / renal absorption / positive regulation of smooth muscle cell migration / positive regulation of calcium ion import / platelet-derived growth factor receptor-beta signaling pathway / 微絨毛 / positive regulation of phosphoprotein phosphatase activity / platelet-derived growth factor receptor signaling pathway / transport across blood-brain barrier / negative regulation of mitotic cell cycle / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / phosphatase binding / 細胞内膜系 / : / positive regulation of calcium-mediated signaling / positive regulation of intrinsic apoptotic signaling pathway / protein-membrane adaptor activity / sperm midpiece / ruffle / lysosomal lumen / cell chemotaxis / Downstream signal transduction / positive regulation of mitotic nuclear division / filopodium / protein localization to plasma membrane / cell periphery / PDZ domain binding / morphogenesis of an epithelium / regulation of actin cytoskeleton organization / brush border membrane / sensory perception of sound / positive regulation of smooth muscle cell proliferation / positive regulation of MAP kinase activity / negative regulation of canonical Wnt signaling pathway / negative regulation of ERK1 and ERK2 cascade / 受容体型チロシンキナーゼ / beta-catenin binding / Wntシグナル経路 / adenylate cyclase-activating dopamine receptor signaling pathway / peptidyl-tyrosine phosphorylation / Constitutive Signaling by Aberrant PI3K in Cancer / positive regulation of reactive oxygen species metabolic process / : 類似検索 - 分子機能 | ||||||
生物種 | HOMO SAPIENS (ヒト) | ||||||
手法 | X線回折 / 分子置換 / 解像度: 2.2 Å | ||||||
データ登録者 | Karthikeyan, S. / Leung, T. / Ladias, J.A.A. | ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 2002 タイトル: Structural Determinants of the Na+/H+ Exchanger Regulatory Factor Interaction with the Beta 2 Adrenergic and Platelet-Derived Growth Factor Receptors 著者: Karthikeyan, S. / Leung, T. / Ladias, J.A.A. #1: ジャーナル: J.Biol.Chem. / 年: 2001 タイトル: Structural Basis of the Na+/H+ Exchanger Regulatory Factor Pdz1 Interaction with the Carboxyl-Terminal Region of the Cystic Fibrosis Transmembrabe Conductance Regulator 著者: Karthikeyan, S. / Leung, T. / Ladias, J.A.A. #2: ジャーナル: J.Mol.Biol. / 年: 2001 タイトル: Crystal Structure of the Pdz1 Domain of Human Na+/ H+ Exchanger Regulatory Factor Provides Insights Into the Mechanism of Carboxyl-Terminal Leucine Recognition by Class I Pdz Domains 著者: Karthikeyan, S. / Leung, T. / Birrane, G. / Webster, G. / Ladias, J.A.A. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1gq5.cif.gz | 30.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1gq5.ent.gz | 19.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1gq5.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/gq/1gq5 ftp://data.pdbj.org/pub/pdb/validation_reports/gq/1gq5 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 9948.382 Da / 分子数: 1 / 断片: PDZ1 DOMAIN, RESIDUES 11-94 / 由来タイプ: 組換発現 / 由来: (組換発現) HOMO SAPIENS (ヒト) / プラスミド: PGEX-2T / 発現宿主: ESCHERICHIA COLI (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: O14745, UniProt: P09619 | ||||
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#2: 化合物 | #3: 水 | ChemComp-HOH / | 配列の詳細 | RESIDUES 9-10 ARE CLONING ARTIFACTS. THE AMINO ACID RESIDUES 95-99 OF PDZ1 CORRESPOND TO THE ...RESIDUES 9-10 ARE CLONING ARTIFACTS. THE AMINO ACID RESIDUES 95-99 OF PDZ1 CORRESPOND | |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.41 Å3/Da / 溶媒含有率: 49 % |
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結晶化 | pH: 4.6 / 詳細: SODIUM ACETATE, SODIUM CHLORIDE, pH 4.60 |
-データ収集
回折 | 平均測定温度: 115 K |
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放射光源 | 由来: 回転陽極 / タイプ: RIGAKU RU300 / 波長: 1.5418 |
検出器 | タイプ: RIGAKU IMAGE PLATE / 検出器: IMAGE PLATE / 日付: 2001年7月3日 / 詳細: MIRRORS |
放射 | モノクロメーター: NI FILTER / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2.2→26.4 Å / Num. obs: 4966 / % possible obs: 95.1 % / Observed criterion σ(I): -3 / 冗長度: 3.4 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 11.8 |
反射 シェル | 解像度: 2.2→2.28 Å / 冗長度: 2.1 % / Rmerge(I) obs: 0.36 / Mean I/σ(I) obs: 2.9 / % possible all: 85.6 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: PDB ENTRY 1I92 解像度: 2.2→26.4 Å / SU B: 8.267 / SU ML: 0.203 / 交差検証法: THROUGHOUT / ESU R: 0.325 / ESU R Free: 0.234
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原子変位パラメータ | Biso mean: 33.1 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2.2→26.4 Å
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