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Yorodumi- PDB-1glh: CATION BINDING TO A BACILLUS (1,3-1,4)-BETA-GLUCANASE. GEOMETRY, ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1glh | ||||||
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Title | CATION BINDING TO A BACILLUS (1,3-1,4)-BETA-GLUCANASE. GEOMETRY, AFFINITY AND EFFECT ON PROTEIN STABILITY | ||||||
Components | 1,3-1,4-BETA-GLUCANASE | ||||||
Keywords | HYDROLASE | ||||||
Function / homology | Function and homology information | ||||||
Biological species | synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Keitel, T. / Heinemann, U. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 1994 Title: Cation binding to a Bacillus (1,3-1,4)-beta-glucanase. Geometry, affinity and effect on protein stability Authors: Keitel, T. / Meldgaard, M. / Heinemann, U. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1993 Title: Molecular and Active-Site Structure of a Bacillus 1,3-1,4-Beta-Glucanase Authors: Keitel, T. / Simon, O. / Borriss, R. / Heinemann, U. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1glh.cif.gz | 53.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1glh.ent.gz | 41.8 KB | Display | PDB format |
PDBx/mmJSON format | 1glh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gl/1glh ftp://data.pdbj.org/pub/pdb/validation_reports/gl/1glh | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 201 |
-Components
#1: Protein | Mass: 23935.230 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli) / References: UniProt: P23904, licheninase |
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#2: Chemical | ChemComp-NA / |
#3: Water | ChemComp-HOH / |
Nonpolymer details | ATOMS IN SODIUM COORDINATION SPHERE: O PRO 9 O GLY 45 O ASP 207 OD1 ASP 207 OH TYR 56 FROM A ...ATOMS IN SODIUM COORDINATI |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.66 Å3/Da / Density % sol: 53.73 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop / Details: Keitel, T., (1991) J. Mol. Biol, 218, 703. | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 13475 / % possible obs: 80.9 % / Observed criterion σ(I): 1 |
-Processing
Software | Name: PROFFT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2→8 Å / σ(F): 1 /
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Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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Software | *PLUS Name: PROFFT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.198 / Rfactor Rfree: 0.225 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |