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- PDB-1gky: REFINED STRUCTURE OF THE COMPLEX BETWEEN GUANYLATE KINASE AND ITS... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1gky | ||||||
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Title | REFINED STRUCTURE OF THE COMPLEX BETWEEN GUANYLATE KINASE AND ITS SUBSTRATE GMP AT 2.0 ANGSTROMS RESOLUTION | ||||||
![]() | GUANYLATE KINASE | ||||||
![]() | TRANSFERASE | ||||||
Function / homology | ![]() Azathioprine ADME / GDP biosynthetic process / guanylate kinase / purine nucleotide metabolic process / Interconversion of nucleotide di- and triphosphates / guanylate kinase activity / phosphorylation / ATP binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Stehle, T. / Schulz, G.E. | ||||||
![]() | ![]() Title: Refined structure of the complex between guanylate kinase and its substrate GMP at 2.0 A resolution. Authors: Stehle, T. / Schulz, G.E. #1: ![]() Title: Three-Dimensional Structure of the Complex of Guanylate Kinase from Yeast with its Substrate Gmp Authors: Stehle, T. / Schulz, G.E. #2: ![]() Title: Guanylate Kinase from Saccharomyces Cerevisiae. Isolation and Characterization, Crystallization and Preliminary X-Ray Analysis, Amino Acid Sequence and Comparison with Adenylate Kinases Authors: Berger, A. / Schiltz, E. / Schulz, G.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 53.3 KB | Display | ![]() |
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PDB format | ![]() | 38 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 779.6 KB | Display | ![]() |
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Full document | ![]() | 781.4 KB | Display | |
Data in XML | ![]() | 11.3 KB | Display | |
Data in CIF | ![]() | 16 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20559.191 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() References: UniProt: P15454, guanylate kinase |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-5GP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.51 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 20 ℃ / pH: 5.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2 Å / Num. obs: 14130 / % possible obs: 100 % / Rmerge(I) obs: 0.05 |
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Processing
Software | Name: SIMULATED / Version: ANNEALING METHOD / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor Rwork: 0.173 / Highest resolution: 2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 7 Å / Rfactor obs: 0.173 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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