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基本情報
登録情報 | データベース: PDB / ID: 1gjj | ||||||
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タイトル | N-TERMINAL CONSTANT REGION OF THE NUCLEAR ENVELOPE PROTEIN LAP2 | ||||||
![]() | LAP2 | ||||||
![]() | MEMBRANE PROTEIN / INNER NUCLEAR MEMBRANE PROTEIN / LAMIN-ASSOCIATED POLYPEPTIDE / LEM DOMAIN / MULTIDIMENSIONAL NMR DIPOLAR COUPLINGS | ||||||
機能・相同性 | ![]() | ||||||
生物種 | ![]() | ||||||
手法 | 溶液NMR / RESTRAINED SIMULATED IN TORSION ANGLE SPACE | ||||||
![]() | Clore, G.M. / Cai, M. | ||||||
![]() | ![]() タイトル: Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM-domain structures: one binds BAF and the other binds DNA. 著者: Cai, M. / Huang, Y. / Ghirlando, R. / Wilson, K.L. / Craigie, R. / Clore, G.M. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 18407.684 Da / 分子数: 1 / 断片: N-TERMINAL CONSTANT REGION (RESIDUES 1-168) / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: 溶液NMR |
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NMR実験の詳細 | Text: THE FOLLOWING EXPERIMENTS WERE CONDUCTED: (1) DOUBLE AND TRIPLE RESONANCE FOR ASSIGNMENT OF PROTEIN; (2) QUANTITATIVE J CORRELATION FOR COUPLING CONSTANTS; (3) 3D SEPARATED NOE EXPERIMENTS; (4) ...Text: THE FOLLOWING EXPERIMENTS WERE CONDUCTED: (1) DOUBLE AND TRIPLE RESONANCE FOR ASSIGNMENT OF PROTEIN; (2) QUANTITATIVE J CORRELATION FOR COUPLING CONSTANTS; (3) 3D SEPARATED NOE EXPERIMENTS; (4) 2D and 3D DOUBLE AND TRIPLE RESONANCE EXPERIMENTS FOR DIPOLAR COUPLING MEASUREMENTS IN LIQUID CRYSTALLINE MEDIUM OF PHAGE PF1. |
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試料調製
試料状態 | イオン強度: 50 mM SODIUM PHOSPHATE / pH: 7.2 / 温度: 308.00 K |
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結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター |
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解析
NMR software | 名称: X-PLOR NIH VERSION (AVAILABLE TO ACADEMIC USERS BY ANONYMOUS FTP AT PORTAL.NIDDK.NIH.GOV IN PUB/CLORE/XPLOR_NIH or AT HTTP://NMR.CIT.NIH.GOV) 開発者: CLORE, KUSZEWSKI AND SCHWIETERS. adapted from Brunger (general XPLOR) 分類: 精密化 |
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精密化 | 手法: RESTRAINED SIMULATED IN TORSION ANGLE SPACE / ソフトェア番号: 1 詳細: THE STRUCTURES WERE CALCULATED BY SIMULATED ANNEALING IN TORSION ANGLE SPACE (C. SCHWIETERS AND G.M. CLORE. IN PRESS). THE TARGET FUNCTION COMPRISES TERMS FOR THE NOE RESTRAINTS, TORSION ...詳細: THE STRUCTURES WERE CALCULATED BY SIMULATED ANNEALING IN TORSION ANGLE SPACE (C. SCHWIETERS AND G.M. CLORE. IN PRESS). THE TARGET FUNCTION COMPRISES TERMS FOR THE NOE RESTRAINTS, TORSION ANGLE RESTRAINTS, CARBON CHEMICAL SHIFT RESTRAINTS (KUSZEWSKI ET AL. J. MAGN. RESON. SERIES B 106, 92-96 (1995)), THE DIPOLAR COUPLING RESTRAINTS (CLORE ET AL. J.MAGN.RESON. 131, 159-162 (1998); J.MAGN.RESON. 133, 216-221(1998)), THE RADIUs OF GYRATION (KUSZEWSKI ET AL. (1999), AND A QUARTIC VAN DER WAALS REPULSION TERM (NILGES ET AL. (1988) FEBS LETT. 229, 129-136). IN THIS ENTRY THE LAST COLUMN REPRESENTS THE AVERAGE RMS DIFFERENCE BETWEEN THE INDIVIDUAL SIMULATED ANNEALING STRUCTURES AND THE MEAN COORDINATE POSITIONS. ONLY COORDINATES FOR RESIDUES 1-50 (LAP2-N) and 111-153 (LAP2-C) ARE PROVIDED. THE LINKER CONNECTING THESE TWO DOMAINS IS COMPLETELY DISORDERED. LIKEWISE THE C-TERMINAL RESIDUES (154-168) ARE DISORDERED. SINCE THE TWO DOMAINS, LAP2-N AND LAP2-C, REORIENT ESSENTIALLY INDEPENDENTLY IN SOLUTION, THE COORDINATES OF THE TWO DOMAINS HAVE BEEN BEST-FITTED TO EACH OTHER SINCE THEY ARE STRUCTURALY VERY SIMILAR. THE LAP2-N DOMAIN BINDS DNA. THE LAP2-C DOMAIN BINDS THE BARRIER-TO-AUTOINTEGRATION FACTOR BAF. Structural Statistics: --------------------------------------------------------- LAP2-N LAP2-C --------------------------------------------------------- DEVIATIONS FROM IDEALIZED GEOMETRY: BONDS 0.003 A 0.003 A ANGLES 0.48 DEG 0.54 DEG IMPROPERS 0.49 DEG 0.39 DEG DEVIATIONS FROM EXPT RESTRAINTS (LAP2-N/LAP2-C) NOES (395/401) 0.024 A 0.012 A TORSION ANGLES (151/121) 0.49 DEG 0.13 DEG 13C CHEMICAL SHIFTS (105/91) 0.92 PPM 0.81 PPM DIPOLAR COUPLING R-FACTORS (CLORE AND GARRETT (1999) J. AM. CHEM. SOC. 121, 9008-9012): 1DNH (39/34) 6.4% 2.9% 1DCH (36/29) 6.4% 1.9% 1DNC' (24/19) 28.2% 26.2% 2DHNC' (24/20) 30.1% 25.3% % RESIDUES IN MOST FAVORABLE REGION OF RAMACHADRAN MAP 84.1% 89.0% NOTE: THE ALIGNMENT TENSOR FOR LAP2-N and LAP2-C ARE DIFFERENT DUE TO THE FACT THAT THE TWO DOMAINS ARE ORIENTED INDEPENDENTLY BY THE PHAGE LIQUID CRYSTALLINE MEDIUM. |
NMRアンサンブル | コンフォーマー選択の基準: RESTRAINED REGULARIZED MEAN STRUCTURE 計算したコンフォーマーの数: 20 / 登録したコンフォーマーの数: 1 |