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- PDB-1gh2: Crystal structure of the catalytic domain of a new human thioredo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1gh2 | ||||||
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Title | Crystal structure of the catalytic domain of a new human thioredoxin-like protein | ||||||
![]() | THIOREDOXIN-LIKE PROTEIN | ||||||
![]() | ELECTRON TRANSPORT / Redox-active center | ||||||
Function / homology | ![]() disulfide oxidoreductase activity / RND1 GTPase cycle / RHOBTB1 GTPase cycle / RND2 GTPase cycle / RND3 GTPase cycle / RHOV GTPase cycle / RHOU GTPase cycle / protein-disulfide reductase activity / RHOBTB2 GTPase cycle / proteasome complex ...disulfide oxidoreductase activity / RND1 GTPase cycle / RHOBTB1 GTPase cycle / RND2 GTPase cycle / RND3 GTPase cycle / RHOV GTPase cycle / RHOU GTPase cycle / protein-disulfide reductase activity / RHOBTB2 GTPase cycle / proteasome complex / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Jin, J. / Chen, X. / Guo, Q. / Yuan, J. / Qiang, B. / Rao, Z. | ||||||
![]() | ![]() Title: Crystal structure of the catalytic domain of a human thioredoxin-like protein. Authors: Jin, J. / Chen, X. / Zhou, Y. / Bartlam, M. / Guo, Q. / Liu, Y. / Sun, Y. / Gao, Y. / Ye, S. / Li, G. / Rao, Z. / Qiang, B. / Yuan, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 32.2 KB | Display | ![]() |
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PDB format | ![]() | 21.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11628.187 Da / Num. of mol.: 1 / Fragment: RESIDUES 2 - 108, CATALYTIC N-TERMINAL DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.12 % |
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Crystal grow | Temperature: 291 K / Method: evaporation / pH: 5 Details: ammonium sulfate, pH 5.0, EVAPORATION, temperature 291.0K |
Crystal grow | *PLUS Method: vapor diffusion, hanging drop |
Components of the solutions | *PLUS Common name: ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 115 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Aug 28, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.22→100 Å / Num. all: 6184 / Num. obs: 6170 / % possible obs: 97.8 % / Observed criterion σ(I): -3 / Redundancy: 7.2 % / Biso Wilson estimate: 20.7 Å2 / Rmerge(I) obs: 0.089 / Net I/σ(I): 8.4 |
Reflection shell | Resolution: 2.22→2.3 Å / Redundancy: 6 % / Rmerge(I) obs: 0.316 / Num. unique all: 624 / % possible all: 98.7 |
Reflection | *PLUS Num. obs: 6710 / % possible obs: 99.8 % |
Reflection shell | *PLUS % possible obs: 98.7 % / Num. unique obs: 624 |
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Processing
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Refinement | Resolution: 2.22→15 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber / Details: CNS
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Refinement step | Cycle: LAST / Resolution: 2.22→15 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Rfactor Rwork: 0.222 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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