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- PDB-1gdt: CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RES... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1gdt | ||||||
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Title | CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE | ||||||
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![]() | DNA BINDING PROTEIN/DNA / PROTEIN-DNA COMPLEX / DOUBLE HELIX / OVERHANGING BASE / DNA BINDING PROTEIN-DNA COMPLEX | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Yang, W. / Steitz, T.A. | ||||||
![]() | ![]() Title: Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site. Authors: Yang, W. / Steitz, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 118.2 KB | Display | ![]() |
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PDB format | ![]() | 89.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 396.2 KB | Display | ![]() |
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Full document | ![]() | 412.1 KB | Display | |
Data in XML | ![]() | 11.1 KB | Display | |
Data in CIF | ![]() | 17.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 6774.416 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||||||||
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#2: DNA chain | Mass: 3966.597 Da / Num. of mol.: 2 / Source method: obtained synthetically #3: DNA chain | | Mass: 6445.209 Da / Num. of mol.: 1 / Source method: obtained synthetically #4: Protein | Mass: 20394.465 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #5: Water | ChemComp-HOH / | Compound details | RESOLVASE GENE IS PUT UNDER THE CONTROL OF THE LAMBDA PROMOTER AND INDUCED BY NALIDIXIC ACID. | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 54 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 54 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 113 K |
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Detector | Date: Apr 1, 1994 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3→30 Å / Num. obs: 13255 / % possible obs: 94.2 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.077 |
Reflection | *PLUS Highest resolution: 3 Å / Lowest resolution: 30 Å / % possible obs: 94.2 % / Observed criterion σ(I): 0 |
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Processing
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Refinement | Resolution: 3→10 Å / σ(F): 2
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Displacement parameters | Biso mean: 26.69 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 3 Å / Lowest resolution: 10 Å / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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