+
Open data
-
Basic information
Entry | Database: PDB / ID: 1gcu | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE OF RAT BILIVERDIN REDUCTASE AT 1.4 A | ||||||
![]() | BILIVERDIN REDUCTASE A | ||||||
![]() | OXIDOREDUCTASE / biliverdin / Rossmann fold | ||||||
Function / homology | ![]() biliverdin reductase / biliberdin reductase (NAD+) activity / biliverdin reductase (NADP+) activity / biliverdin reductase [NAD(P)+] activity / Heme degradation / Cytoprotection by HMOX1 / heme catabolic process / nucleotide binding / zinc ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kikuchi, A. / Park, S.Y. / Shiro, Y. | ||||||
![]() | ![]() Title: Crystal structure of rat biliverdin reductase. Authors: Kikuchi, A. / Park, S.Y. / Miyatake, H. / Sun, D. / Sato, M. / Yoshida, T. / Shiro, Y. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 75.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 55.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 368.4 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 372.7 KB | Display | |
Data in XML | ![]() | 7.2 KB | Display | |
Data in CIF | ![]() | 12 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 33611.535 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 54.49 % | |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 283 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: (NH4)2SO4, Na citrate, Na/K tartrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K | |||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 51 % | |||||||||||||||||||||||||
Crystal grow | *PLUS | |||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Mar 20, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.7 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→32.71 Å / Num. all: 379020 / Num. obs: 379020 / Observed criterion σ(F): 0 / Redundancy: 5.3 % / Biso Wilson estimate: 18.3 Å2 / Rmerge(I) obs: 0.079 / Net I/σ(I): 6 |
Reflection shell | Resolution: 1.4→1.48 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.324 / Num. unique all: 9812 / % possible all: 94.3 |
Reflection | *PLUS Num. obs: 71182 / Num. measured all: 379020 |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.4→32.7 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.4→32.7 Å
| |||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||
Software | *PLUS Name: ![]() | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.4 Å / Lowest resolution: 32.7 Å / σ(F): 0 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 1.6 |