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- PDB-1g7s: X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLE... -

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Basic information

Entry
Database: PDB / ID: 1g7s
TitleX-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLEXED WITH GDP
ComponentsTRANSLATION INITIATION FACTOR IF2/EIF5B
KeywordsTRANSLATION / translational GTPase
Function / homology
Function and homology information


translation initiation factor activity / GTPase activity / GTP binding / cytoplasm
Similarity search - Function
Translation initiation factor IF- 2, domain 3 / Translation initiation factor aIF-2, archaea / Elongation factor Tu-type domain / Elongation factor Tu domain 4 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / Translation factors / Translation elongation factor EFTu-like, domain 2 ...Translation initiation factor IF- 2, domain 3 / Translation initiation factor aIF-2, archaea / Elongation factor Tu-type domain / Elongation factor Tu domain 4 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / Translation factors / Translation elongation factor EFTu-like, domain 2 / Elongation factor Tu domain 2 / Elongation Factor Tu (Ef-tu); domain 3 / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Small GTP-binding protein domain / Translation protein, beta-barrel domain superfamily / P-loop containing nucleotide triphosphate hydrolases / Beta Barrel / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / Probable translation initiation factor IF-2
Similarity search - Component
Biological speciesMethanothermobacter thermautotrophicus (archaea)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2 Å
AuthorsRoll-Mecak, A. / Cao, C. / Dever, T.E. / Burley, S.K.
CitationJournal: Cell(Cambridge,Mass.) / Year: 2000
Title: X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding.
Authors: Roll-Mecak, A. / Cao, C. / Dever, T.E. / Burley, S.K.
History
DepositionNov 14, 2000Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 6, 2000Provider: repository / Type: Initial release
Revision 1.1Apr 27, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 3, 2021Group: Database references / Derived calculations / Structure summary
Category: audit_author / citation_author / struct_site
Item: _audit_author.identifier_ORCID / _citation_author.identifier_ORCID ..._audit_author.identifier_ORCID / _citation_author.identifier_ORCID / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Revision 1.4Feb 7, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_sheet
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_sheet.number_strands

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: TRANSLATION INITIATION FACTOR IF2/EIF5B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)66,7842
Polymers66,3401
Non-polymers4431
Water8,053447
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)48.430, 54.523, 91.860
Angle α, β, γ (deg.)104.54, 100.18, 99.04
Int Tables number1
Space group name H-MP1

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Components

#1: Protein TRANSLATION INITIATION FACTOR IF2/EIF5B


Mass: 66340.391 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Methanothermobacter thermautotrophicus (archaea)
Production host: Escherichia coli (E. coli) / References: UniProt: O26359
#2: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 447 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 2

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Sample preparation

CrystalDensity Matthews: 3.4 Å3/Da / Density % sol: 63.86 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.8
Details: 0.1M CAcodylate pH 5.8, 18% PEG4000, 0.2M Lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Crystal grow
*PLUS
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-IDChemical formula
115 mg/mlprotein1drop
20.2 M1dropLiSO4
3100 mMcacodylate1reservoir
418 %PEG40001reservoir

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Data collection

DiffractionMean temperature: 95 K
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 0.9788 Å
DetectorType: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 28, 2000
RadiationMonochromator: graphite / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9788 Å / Relative weight: 1
ReflectionResolution: 2→22 Å / Num. all: 58005 / Num. obs: 53169 / % possible obs: 90 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 6 % / Rmerge(I) obs: 0.058 / Net I/σ(I): 25
Reflection shellResolution: 2→2.05 Å / Redundancy: 6 % / Rmerge(I) obs: 0.15 / % possible all: 86.4
Reflection shell
*PLUS
% possible obs: 86.4 %

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Processing

Software
NameClassification
SHARPphasing
CNSrefinement
DENZOdata reduction
SCALEPACKdata scaling
RefinementMethod to determine structure: MAD / Resolution: 2→22 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber
Details: In REMARK 500, the covalent bonds which deviate from the dictionary values by more than 6RMSD are from residues for which the electron density is very poor beyond (and including) the CB atom.
RfactorNum. reflectionSelection details
Rfree0.265 5419 10% of data
Rwork0.22 --
all-59085 -
obs-55816 -
Displacement parameters
Baniso -1Baniso -2Baniso -3
1-0.067 Å2-0.903 Å2-7.777 Å2
2--6.12 Å2-3.416 Å2
3----6.186 Å2
Refinement stepCycle: LAST / Resolution: 2→22 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4401 0 28 447 4876
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONx_bond_d0.009
X-RAY DIFFRACTIONx_angle_deg1.5
LS refinement shellResolution: 2→2.01 Å /
RfactorNum. reflection
Rfree0.179 22
Rwork0.168 -
obs-215
Software
*PLUS
Name: CNS / Classification: refinement
Refine LS restraints
*PLUS
Refine-IDType
X-RAY DIFFRACTIONc_bond_d
X-RAY DIFFRACTIONc_angle_deg

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