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- PDB-1g5m: HUMAN BCL-2, ISOFORM 1 -

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Entry
Database: PDB / ID: 1g5m
TitleHUMAN BCL-2, ISOFORM 1
ComponentsPROTEIN (APOPTOSIS REGULATOR BCL-2 WITH PUTATIVE FLEXIBLE LOOP REPLACED WITH A PORTION OF APOPTOSIS REGULATOR BCL-X PROTEIN)
KeywordsAPOPTOSIS
Function / homology
Function and homology information


BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members / Activation of BAD and translocation to mitochondria / Interleukin-4 and Interleukin-13 signaling / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / Estrogen-dependent gene expression / The NLRP1 inflammasome / CD8-positive, alpha-beta T cell lineage commitment / pigment granule organization / negative regulation of cellular pH reduction / negative regulation of retinal cell programmed cell death ...BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members / Activation of BAD and translocation to mitochondria / Interleukin-4 and Interleukin-13 signaling / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / Estrogen-dependent gene expression / The NLRP1 inflammasome / CD8-positive, alpha-beta T cell lineage commitment / pigment granule organization / negative regulation of cellular pH reduction / negative regulation of retinal cell programmed cell death / channel inhibitor activity / positive regulation of skeletal muscle fiber development / positive regulation of melanocyte differentiation / regulation of glycoprotein biosynthetic process / melanin metabolic process / cochlear nucleus development / negative regulation of osteoblast proliferation / mesenchymal cell development / regulation of cell-matrix adhesion / glomerulus development / oocyte development / lymphoid progenitor cell differentiation / negative regulation of calcium ion transport into cytosol / gland morphogenesis / renal system process / positive regulation of neuron maturation / melanocyte differentiation / apoptotic process in bone marrow cell / ear development / regulation of protein homodimerization activity / channel activity / negative regulation of mitochondrial depolarization / positive regulation of multicellular organism growth / negative regulation of ossification / metanephros development / cellular process regulating host cell cycle in response to virus / regulation of protein heterodimerization activity / negative regulation of myeloid cell apoptotic process / endoplasmic reticulum calcium ion homeostasis / negative regulation of protein localization to plasma membrane / B cell proliferation / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage / organ growth / response to UV-B / regulation of nitrogen utilization / fertilization / regulation of viral genome replication / negative regulation of execution phase of apoptosis / suppression by virus of host apoptotic process / focal adhesion assembly / intrinsic apoptotic signaling pathway in response to oxidative stress / BH3 domain binding / mitochondrion morphogenesis / Bcl-2 family protein complex / response to iron ion / response to cycloheximide / cellular response to alkaloid / B cell lineage commitment / negative regulation of G1/S transition of mitotic cell cycle / digestive tract morphogenesis / axon regeneration / apoptotic mitochondrial changes / hair follicle morphogenesis / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of growth / mitotic cell cycle checkpoint / branching involved in ureteric bud morphogenesis / humoral immune response / positive regulation of smooth muscle cell migration / behavioral fear response / T cell homeostasis / pore complex / germ cell development / hepatocyte apoptotic process / negative regulation of apoptotic signaling pathway / negative regulation of release of cytochrome c from mitochondria / extrinsic apoptotic signaling pathway via death domain receptors / regulation of calcium ion transport / regulation of mitochondrial membrane permeability / positive regulation of catalytic activity / B cell homeostasis / reactive oxygen species metabolic process / regulation of transmembrane transporter activity / cellular response to glucose starvation / homeostasis of number of cells within a tissue / negative regulation of anoikis / negative regulation of reactive oxygen species metabolic process / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / positive regulation of B cell proliferation / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / release of cytochrome c from mitochondria / negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / actin filament organization / ovarian follicle development / positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway / negative regulation of intrinsic apoptotic signaling pathway / positive regulation of intrinsic apoptotic signaling pathway / post-embryonic development / negative regulation of cell migration / cell aging
Blc2-like superfamily / Apoptosis regulator, Bcl-2 protein, BH4 / Apoptosis regulator proteins, Bcl-2 family / Apoptosis regulator, Bcl-2/ BclX / Blc2 family / Apoptosis regulator, Bcl-2, BH2 motif, conserved site / Bcl-2 homology region 4 / Apoptosis regulator, Bcl-2 family BH1 motif signature. / Apoptosis regulator, Bcl-2 family BH2 motif signature. / Apoptosis regulator, Bcl-2 family BH3 motif signature. ...Blc2-like superfamily / Apoptosis regulator, Bcl-2 protein, BH4 / Apoptosis regulator proteins, Bcl-2 family / Apoptosis regulator, Bcl-2/ BclX / Blc2 family / Apoptosis regulator, Bcl-2, BH2 motif, conserved site / Bcl-2 homology region 4 / Apoptosis regulator, Bcl-2 family BH1 motif signature. / Apoptosis regulator, Bcl-2 family BH2 motif signature. / Apoptosis regulator, Bcl-2 family BH3 motif signature. / Apoptosis regulator, Bcl-2 family BH4 motif signature. / BCL2-like apoptosis inhibitors family profile. / Apoptosis regulator, Bcl-2 family BH4 motif profile. / Bcl2-like / Apoptosis regulator, Bcl-2, BH4 motif, conserved site / Apoptosis regulator, Bcl-2, BH3 motif, conserved site / Apoptosis regulator, Bcl-2 / Apoptosis regulator, Bcl-2, BH1 motif, conserved site / Apoptosis regulator, Bcl-X
Apoptosis regulator Bcl-2 / Bcl-2-like protein 1
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR / simulated annealing
AuthorsPetros, A.M. / Medek, A. / Nettesheim, D.G. / Kim, D.H. / Yoon, H.S. / Swift, K. / Matayoshi, E.D. / Oltersdorf, T. / Fesik, S.W.
Citation
Journal: Proc.Natl.Acad.Sci.USA / Year: 2001
Title: Solution structure of the antiapoptotic protein bcl-2.
Authors: Petros, A.M. / Medek, A. / Nettesheim, D.G. / Kim, D.H. / Yoon, H.S. / Swift, K. / Matayoshi, E.D. / Oltersdorf, T. / Fesik, S.W.
#1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1986
Title: Analysis of the structure, transcripts, and products of bcl-2, the gene involved in human follicular lymphoma
Authors: Tsujimoto, Y. / Croce, C.M.
Validation Report
SummaryFull reportAbout validation report
History
DepositionNov 1, 2000Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 21, 2001Provider: repository / Type: Initial release
Revision 1.1Apr 27, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: PROTEIN (APOPTOSIS REGULATOR BCL-2 WITH PUTATIVE FLEXIBLE LOOP REPLACED WITH A PORTION OF APOPTOSIS REGULATOR BCL-X PROTEIN)


Theoretical massNumber of molelcules
Total (without water)19,2271
Polymers19,2271
Non-polymers00
Water0
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: Protein/peptide PROTEIN (APOPTOSIS REGULATOR BCL-2 WITH PUTATIVE FLEXIBLE LOOP REPLACED WITH A PORTION OF APOPTOSIS REGULATOR BCL-X PROTEIN)


Mass: 19227.389 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: P10415, UniProt: Q07817

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment

Conditions-ID: 1

Experiment-IDSolution-IDType
113D 15N-separated NOESY
223D 13C-separated NOESY

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Sample preparation

Details
Solution-IDContentsSolvent system
115N-Bcl-2(1); 15N,13C-BCL-2(1)H2O
215N,13C-Bcl-2(1)D2O
Sample conditionsIonic strength: 20 mM / pH: 7.8 / Pressure: ambient / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometer

Manufacturer: Bruker / Model: Avance / Type: Bruker Avance

Field strength (MHz)Spectrometer-ID
8001
6002
5003

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Processing

NMR softwareName: CNX / Version: 2000 / Developer: Brunger / Classification: refinement
RefinementMethod: simulated annealing / Software ordinal: 1
NMR ensembleConformers submitted total number: 1

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