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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1g2z | ||||||
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タイトル | DIMERIZATION DOMAIN OF HNF-1ALPHA WITH A LEU 13 SELENOMETHIONINE SUBSTITUTION | ||||||
![]() | HEPATOCYTE NUCLEAR FACTOR 1-ALPHA | ||||||
![]() | TRANSCRIPTION / dimerization domain / four-helix bundle / transcription factor / selenomethionine | ||||||
機能・相同性 | ![]() paraxial mesoderm formation / cellular response to rapamycin / apoptotic nuclear changes / regulation of NADP metabolic process / renal glucose absorption / regulation of hormone secretion / reproductive structure development / cellular response to L-leucine / bile acid biosynthetic process / reverse cholesterol transport ...paraxial mesoderm formation / cellular response to rapamycin / apoptotic nuclear changes / regulation of NADP metabolic process / renal glucose absorption / regulation of hormone secretion / reproductive structure development / cellular response to L-leucine / bile acid biosynthetic process / reverse cholesterol transport / pronucleus / bile acid and bile salt transport / regulation of Wnt signaling pathway / pancreas development / negative regulation of miRNA processing / positive regulation of mitochondrial membrane potential / embryonic limb morphogenesis / insulin secretion / heme biosynthetic process / positive regulation of ATP biosynthetic process / negative regulation of peptidyl-threonine phosphorylation / glucose import / blastocyst development / photoreceptor outer segment / fatty acid transport / response to glucose / bone resorption / cholesterol metabolic process / liver development / transcription coregulator binding / cellular response to glucose stimulus / placenta development / protein localization / positive regulation of insulin secretion / fatty acid biosynthetic process / transcription coactivator binding / glucose homeostasis / double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / response to oxidative stress / transcription regulator complex / sequence-specific DNA binding / transcription by RNA polymerase II / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / protein dimerization activity / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin remodeling / positive regulation of protein phosphorylation / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / chromatin binding / positive regulation of gene expression / regulation of DNA-templated transcription / chromatin / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / identical protein binding / nucleus / cytoplasm 類似検索 - 分子機能 | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Rose, R.B. / Endrizzi, J.A. / Cronk, J.D. / Holton, J. / Alber, T. | ||||||
![]() | ![]() タイトル: High-resolution structure of the HNF-1alpha dimerization domain. 著者: Rose, R.B. / Endrizzi, J.A. / Cronk, J.D. / Holton, J. / Alber, T. #1: ![]() タイトル: Structural basis of dimerization, coactivator recognition and MODY3 mutations in HNF-1alpha 著者: Rose, R.B. / Bayle, J.H. / Endrizzi, J.A. / Cronk, J.D. / Crabtree, G.R. / Alber, T. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 24.4 KB | 表示 | ![]() |
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PDB形式 | ![]() | 17.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 417.6 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 418.5 KB | 表示 | |
XML形式データ | ![]() | 6.3 KB | 表示 | |
CIF形式データ | ![]() | 8.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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1 | ![]()
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単位格子 |
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詳細 | Two monomers are in the asymmetric unit, although not the biologically relevant dimer. The dimer of each of the monomers is generated by the two fold axis: -x+2, -y, z. |
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要素
#1: タンパク質・ペプチド | 分子量: 3451.884 Da / 分子数: 2 / 断片: DIMERIZATION DOMAIN, RESIDUES 1-32 / 変異: L13(MSE) / 由来タイプ: 合成 詳細: This peptide was chemically synthesized. The sequence of this peptide naturally occurs in mouse (Mus musculus), with a point mutation at position 13. 参照: UniProt: P22361 #2: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.33 Å3/Da / 溶媒含有率: 47.22 % | ||||||||||||||||||||||||||||||
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結晶化 | 温度: 277 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8.5 詳細: PEG 4000, Tris-HCl, lithium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K | ||||||||||||||||||||||||||||||
結晶化 | *PLUS 手法: 蒸気拡散法 | ||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 200 K | |||||||||||||||
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放射光源 | 由来: ![]() ![]() ![]() | |||||||||||||||
検出器 | タイプ: ADSC QUANTUM 4 / 検出器: CCD / 日付: 1999年10月28日 / 詳細: Double crystal | |||||||||||||||
放射 | モノクロメーター: double crystal / プロトコル: MAD / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray | |||||||||||||||
放射波長 |
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反射 | 解像度: 1.15→29.5 Å / Num. all: 22638 / Num. obs: 22638 / % possible obs: 96 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / 冗長度: 4.4 % / Biso Wilson estimate: 10.3 Å2 / Rmerge(I) obs: 0.065 / Net I/σ(I): 16.2 | |||||||||||||||
反射 シェル | 解像度: 1.15→1.19 Å / 冗長度: 2.9 % / Rmerge(I) obs: 0.18 / Mean I/σ(I) obs: 5.7 / Num. unique all: 2032 / % possible all: 90 | |||||||||||||||
反射 | *PLUS Num. obs: 31540 / % possible obs: 93 % / 冗長度: 4.3 % / Rmerge(I) obs: 0.066 | |||||||||||||||
反射 シェル | *PLUS 最高解像度: 1.05 Å / 最低解像度: 1.11 Å / % possible obs: 82.1 % / Rmerge(I) obs: 0.398 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: wARP model 解像度: 1.15→29.5 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 563662.01 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: Engh & Huber 詳細: Refinement carried out in tnt. Statistics reported from CNS (input file: model_stats.list).
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溶媒の処理 | 溶媒モデル: FLAT MODEL / Bsol: 97.28 Å2 / ksol: 0.4 e/Å3 | ||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 31.2 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 1.15→29.5 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 1.15→1.19 Å / Rfactor Rfree error: 0.007 / Total num. of bins used: 6
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Xplor file |
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ソフトウェア | *PLUS 名称: CNS / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS σ(F): 0 / Num. reflection Rfree: 1443 / % reflection Rfree: 5 % / Rfactor obs: 0.223 / Rfactor Rfree: 0.253 | ||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS Biso mean: 31.2 Å2 | ||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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LS精密化 シェル | *PLUS Rfactor Rfree: 0.31 / % reflection Rfree: 5.3 % / Rfactor Rwork: 0.35 |