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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1fzd | |||||||||
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タイトル | STRUCTURE OF RECOMBINANT ALPHAEC DOMAIN FROM HUMAN FIBRINOGEN-420 | |||||||||
![]() | FIBRINOGEN-420 | |||||||||
![]() | BLOOD COAGULATION / FIBRINOGEN-420 / ALPHAEC DOMAIN / FIBRINOGEN RELATED DOMAIN / GLYCOSYLATED PROTEIN | |||||||||
機能・相同性 | ![]() blood coagulation, common pathway / induction of bacterial agglutination / fibrinogen complex / Regulation of TLR by endogenous ligand / platelet alpha granule / blood coagulation, fibrin clot formation / MyD88 deficiency (TLR2/4) / positive regulation of heterotypic cell-cell adhesion / IRAK4 deficiency (TLR2/4) / MyD88:MAL(TIRAP) cascade initiated on plasma membrane ...blood coagulation, common pathway / induction of bacterial agglutination / fibrinogen complex / Regulation of TLR by endogenous ligand / platelet alpha granule / blood coagulation, fibrin clot formation / MyD88 deficiency (TLR2/4) / positive regulation of heterotypic cell-cell adhesion / IRAK4 deficiency (TLR2/4) / MyD88:MAL(TIRAP) cascade initiated on plasma membrane / extracellular matrix structural constituent / plasminogen activation / p130Cas linkage to MAPK signaling for integrins / positive regulation of peptide hormone secretion / positive regulation of exocytosis / GRB2:SOS provides linkage to MAPK signaling for Integrins / protein polymerization / Integrin cell surface interactions / negative regulation of endothelial cell apoptotic process / Common Pathway of Fibrin Clot Formation / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / positive regulation of substrate adhesion-dependent cell spreading / positive regulation of vasoconstriction / fibrinolysis / Integrin signaling / cell-matrix adhesion / platelet alpha granule lumen / positive regulation of protein secretion / Post-translational protein phosphorylation / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / platelet aggregation / response to calcium ion / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / Signaling by BRAF and RAF1 fusions / extracellular vesicle / Platelet degranulation / ER-Phagosome pathway / protein-containing complex assembly / collagen-containing extracellular matrix / adaptive immune response / positive regulation of ERK1 and ERK2 cascade / blood microparticle / Amyloid fiber formation / endoplasmic reticulum lumen / external side of plasma membrane / innate immune response / signaling receptor binding / structural molecule activity / cell surface / endoplasmic reticulum / extracellular space / extracellular exosome / extracellular region / metal ion binding / plasma membrane 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | ![]() ![]() ![]() | |||||||||
![]() | Spraggon, G. / Applegate, D. / Everse, S.J. / Zhang, J.-Z. / Veerapandian, L. / Redman, C. / Doolittle, R.F. / Grieninger, G. | |||||||||
![]() | ![]() タイトル: Crystal structure of a recombinant alphaEC domain from human fibrinogen-420. 著者: Spraggon, G. / Applegate, D. / Everse, S.J. / Zhang, J.Z. / Veerapandian, L. / Redman, C. / Doolittle, R.F. / Grieninger, G. | |||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 337.3 KB | 表示 | ![]() |
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PDB形式 | ![]() | 279.6 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 912.2 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1009.4 KB | 表示 | |
XML形式データ | ![]() | 46.4 KB | 表示 | |
CIF形式データ | ![]() | 69.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 1fczS S: 精密化の開始モデル |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper:
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要素
-タンパク質 , 1種, 8分子 ABCDEFGH
#1: タンパク質 | 分子量: 22840.660 Da / 分子数: 8 / 断片: ALPHA-EC DOMAIN / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
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-糖 , 4種, 10分子 ![](data/chem/img/NAG.gif)
#2: 多糖 | #3: 多糖 | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | #4: 多糖 | #6: 糖 | ChemComp-NAG / |
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-非ポリマー , 2種, 504分子 ![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: 化合物 | ChemComp-CA / #7: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.58 Å3/Da / 溶媒含有率: 50 % | ||||||||||||||||||||||||||||||||||||||||||
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結晶化 | 手法: 蒸気拡散法, シッティングドロップ法 / pH: 5.5 詳細: PROTEIN WAS CRYSTALLIZED BY SITTING DROP VAPOUR DIFFUSION FROM 20% PEG 3350, 0.15 M CACL2, 0.1M IMIDAZOLE- ACETATE PH 5.5 0.002 M SODIUM AZIDE, vapor diffusion - sitting drop | ||||||||||||||||||||||||||||||||||||||||||
結晶 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS pH: 7.5 / 手法: 蒸気拡散法, シッティングドロップ法 | ||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 293 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | 検出器: CCD / 日付: 1998年2月20日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.08 Å / 相対比: 1 |
反射 | 解像度: 2.1→20 Å / Num. obs: 110459 / % possible obs: 91.2 % / Observed criterion σ(I): 0 / 冗長度: 5.2 % / Rmerge(I) obs: 0.098 |
反射 | *PLUS Num. measured all: 1169328 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 1FCZ (GAMMA DOMAIN) 解像度: 2.1→20 Å / 交差検証法: THROUGHOUT / σ(F): 0 詳細: CARBOHYDRATE RESIDUES 12 - 15 FOR CHAINS A, B, C, AND D WERE MODELLED INTO WEAK ELECTRON DENSITY. THERE WAS NO ELECTRON DENSITY OBSERVED FOR THESE RESIDUES IN CHAINS E, F, G, AND H.
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精密化ステップ | サイクル: LAST / 解像度: 2.1→20 Å
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拘束条件 |
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