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Open data
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Basic information
Entry | Database: PDB / ID: 1fwk | ||||||
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Title | CRYSTAL STRUCTURE OF HOMOSERINE KINASE COMPLEXED WITH ADP | ||||||
![]() | HOMOSERINE KINASE | ||||||
![]() | TRANSFERASE / kinase | ||||||
Function / homology | ![]() homoserine kinase / homoserine kinase activity / threonine biosynthetic process / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Zhou, T. / Daugherty, M. / Grishin, N.V. / Osterman, A.L. / Zhang, H. | ||||||
![]() | ![]() Title: Structure and mechanism of homoserine kinase: prototype for the GHMP kinase superfamily. Authors: Zhou, T. / Daugherty, M. / Grishin, N.V. / Osterman, A.L. / Zhang, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 246.2 KB | Display | ![]() |
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PDB format | ![]() | 199.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Details | The biological assembly is a dimer: chain A and B or chain C and D in the asymmeric unit. |
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Components
#1: Protein | Mass: 32299.371 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #2: Chemical | ChemComp-ADP / #3: Chemical | ChemComp-MG / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.29 Å3/Da / Density % sol: 62.58 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: PEG4000, sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 7.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jul 4, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→40 Å / Num. all: 97265 / Num. obs: 96677 / % possible obs: 99.4 % / Redundancy: 3.3 % / Rmerge(I) obs: 0.042 / Net I/σ(I): 29.5 |
Reflection shell | Resolution: 2.1→2.14 Å / Rmerge(I) obs: 0.026 / Num. unique all: 4826 / % possible all: 99.5 |
Reflection | *PLUS Num. measured all: 316441 |
Reflection shell | *PLUS % possible obs: 99.5 % / Mean I/σ(I) obs: 2.65 |
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Processing
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Refinement | Resolution: 2.1→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Hube
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Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_bond_d / Dev ideal: 0.013 |