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Yorodumi- PDB-1fu0: CRYSTAL STRUCTURE ANALYSIS OF THE PHOSPHO-SERINE 46 HPR FROM ENTE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fu0 | ||||||
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Title | CRYSTAL STRUCTURE ANALYSIS OF THE PHOSPHO-SERINE 46 HPR FROM ENTEROCOCCUS FAECALIS | ||||||
Components | PHOSPHOCARRIER PROTEIN HPR | ||||||
Keywords | SIGNALING PROTEIN / Phospho-Serine HPr / PTS System | ||||||
Function / homology | Function and homology information phosphoenolpyruvate-dependent sugar phosphotransferase system / cytoplasm Similarity search - Function | ||||||
Biological species | Enterococcus faecalis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.9 Å | ||||||
Authors | Audette, G.F. / Engelmann, R. / Hengstenberg, W. / Deutscher, J. / Hayakawa, K. / Quail, J.W. / Delbaere, L.T.J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: The 1.9 A resolution structure of phospho-serine 46 HPr from Enterococcus faecalis. Authors: Audette, G.F. / Engelmann, R. / Hengstenberg, W. / Deutscher, J. / Hayakawa, K. / Quail, J.W. / Delbaere, L.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fu0.cif.gz | 41.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fu0.ent.gz | 31.8 KB | Display | PDB format |
PDBx/mmJSON format | 1fu0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fu0_validation.pdf.gz | 421.7 KB | Display | wwPDB validaton report |
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Full document | 1fu0_full_validation.pdf.gz | 422.6 KB | Display | |
Data in XML | 1fu0_validation.xml.gz | 9.9 KB | Display | |
Data in CIF | 1fu0_validation.cif.gz | 12.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fu/1fu0 ftp://data.pdbj.org/pub/pdb/validation_reports/fu/1fu0 | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.99872, 0.02055, 0.04623), Vector: Details | The biological assembly is a monomer | |
-Components
#1: Protein | Mass: 9281.466 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Enterococcus faecalis (bacteria) / Strain: 26487 / References: UniProt: P07515 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.8 Å3/Da / Density % sol: 31.74 % | ||||||||||||||||||||
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Crystal grow | Temperature: 287 K / Method: vapor diffusion, hanging drop / pH: 4.2 Details: 2.8M sodium-potassium phosphate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 287.0K | ||||||||||||||||||||
Crystal | *PLUS Density % sol: 31.3 % | ||||||||||||||||||||
Crystal grow | *PLUS Details: used seeding | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-18B / Wavelength: 1 |
Detector | Type: WEISSENBERG / Detector: DIFFRACTOMETER / Date: Dec 14, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→40 Å / Num. all: 10043 / Num. obs: 10043 / % possible obs: 95.1 % / Redundancy: 4.23 % / Biso Wilson estimate: 14.8 Å2 / Rmerge(I) obs: 0.041 / Net I/σ(I): 8.6 |
Reflection shell | Resolution: 1.9→1.93 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.11 / Num. unique all: 447 / % possible all: 84.3 |
Reflection | *PLUS Num. measured all: 42471 |
Reflection shell | *PLUS % possible obs: 84.3 % / Num. unique obs: 447 / Num. measured obs: 1668 |
-Processing
Software |
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Refinement | Resolution: 1.9→40 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber / Details: Simulated Annealing
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Refinement step | Cycle: LAST / Resolution: 1.9→40 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 40 Å / σ(F): 0 / % reflection Rfree: 5 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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