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Yorodumi- PDB-1fte: CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE ACYL CARRIER PROTEI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fte | ||||||
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Title | CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE ACYL CARRIER PROTEIN SYNTHASE (NATIVE 1) | ||||||
Components | ACYL CARRIER PROTEIN SYNTHASE | ||||||
Keywords | TRANSFERASE / Acyl Carrier Protein Synthase | ||||||
Function / homology | Function and homology information holo-[acyl-carrier-protein] synthase / holo-[acyl-carrier-protein] synthase activity / fatty acid biosynthetic process / magnesium ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Streptococcus pneumoniae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.4 Å | ||||||
Authors | Chirgadze, N. / Briggs, S. / McAllister, K. / Fischl, A. / Zhao, G. | ||||||
Citation | Journal: EMBO J. / Year: 2000 Title: Crystal structure of Streptococcus pneumoniae acyl carrier protein synthase: an essential enzyme in bacterial fatty acid biosynthesis. Authors: Chirgadze, N.Y. / Briggs, S.L. / McAllister, K.A. / Fischl, A.S. / Zhao, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fte.cif.gz | 79.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fte.ent.gz | 60 KB | Display | PDB format |
PDBx/mmJSON format | 1fte.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fte_validation.pdf.gz | 395.2 KB | Display | wwPDB validaton report |
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Full document | 1fte_full_validation.pdf.gz | 399.8 KB | Display | |
Data in XML | 1fte_validation.xml.gz | 8.4 KB | Display | |
Data in CIF | 1fte_validation.cif.gz | 13.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ft/1fte ftp://data.pdbj.org/pub/pdb/validation_reports/ft/1fte | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13678.506 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus pneumoniae (bacteria) / Production host: Escherichia coli (E. coli) References: UniProt: P0A2W6, holo-[acyl-carrier-protein] synthase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.2 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 294 K / pH: 7.1 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 29, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→20 Å / Num. all: 14842 / Num. obs: 14842 / % possible obs: 96 % / Observed criterion σ(I): 0 / Redundancy: 4 % / Rmerge(I) obs: 0.049 / Net I/σ(I): 25.6 |
Reflection shell | Resolution: 2.4→2.49 Å / % possible obs: 84 % / Rmerge(I) obs: 0.23 / Mean I/σ(I) obs: 4.5 / % possible all: 84 |
Reflection | *PLUS Num. all: 59335 / % possible obs: 96 % / Redundancy: 4 % / Num. measured all: 336325 |
Reflection shell | *PLUS Rmerge(I) obs: 0.23 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.4→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0
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Refinement step | Cycle: LAST / Resolution: 2.4→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.46 Å / Total num. of bins used: 13
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Software | *PLUS Name: CNX / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / σ(F): 0 / % reflection Rfree: 4.9 % / Rfactor obs: 0.229 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS % reflection Rfree: 4.9 % / Rfactor Rwork: 0.309 |