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Yorodumi- PDB-1fsz: CRYSTAL STRUCTURE OF THE CELL-DIVISION PROTEIN FTSZ AT 2.8A RESOLUTION -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fsz | ||||||
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Title | CRYSTAL STRUCTURE OF THE CELL-DIVISION PROTEIN FTSZ AT 2.8A RESOLUTION | ||||||
Components | FTSZ | ||||||
Keywords | CELL-DIVISION PROTEIN / FTSZ / CELL DIVISION PROTEIN / TUBULIN | ||||||
Function / homology | Function and homology information FtsZ-dependent cytokinesis / division septum assembly / cell division site / protein polymerization / cell division / GTPase activity / GTP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Methanocaldococcus jannaschii (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 2.8 Å | ||||||
Authors | Lowe, J. / Amos, L.A. | ||||||
Citation | Journal: Nature / Year: 1998 Title: Crystal structure of the bacterial cell-division protein FtsZ. Authors: Lowe, J. / Amos, L.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fsz.cif.gz | 77.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fsz.ent.gz | 58.2 KB | Display | PDB format |
PDBx/mmJSON format | 1fsz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fsz_validation.pdf.gz | 783.7 KB | Display | wwPDB validaton report |
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Full document | 1fsz_full_validation.pdf.gz | 804.7 KB | Display | |
Data in XML | 1fsz_validation.xml.gz | 17.6 KB | Display | |
Data in CIF | 1fsz_validation.cif.gz | 24.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fs/1fsz ftp://data.pdbj.org/pub/pdb/validation_reports/fs/1fsz | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 39943.121 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanocaldococcus jannaschii (archaea) Description: DSM 2661 METHANOCOCCUS JANNASCHII / Cellular location: CYTOPLASM-MEMBRANE / Cellular location (production host): CYTOPLASM / Production host: Escherichia coli (E. coli) / Strain (production host): C41 / References: UniProt: Q57816 |
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#2: Chemical | ChemComp-GDP / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.2 Å3/Da / Density % sol: 71 % | |||||||||||||||||||||||||
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Crystal grow | pH: 6.62 / Details: pH 6.62 | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.6 / Wavelength: 0.87 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 9, 1997 / Details: MIRRORS |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→37.8 Å / Num. obs: 17024 / % possible obs: 99 % / Redundancy: 3 % / Biso Wilson estimate: 65 Å2 / Rmerge(I) obs: 0.065 / Rsym value: 0.065 / Net I/σ(I): 9.9 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.336 / Mean I/σ(I) obs: 2.9 / Rsym value: 0.336 / % possible all: 99.7 |
Reflection shell | *PLUS % possible obs: 99.7 % / Rmerge(I) obs: 0.31 / Mean I/σ(I) obs: 2.3 |
-Processing
Software |
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Refinement | Method to determine structure: MIRAS / Resolution: 2.8→8 Å / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.0001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: USED REFMAC UP TO 3.5 ANGSTROMS RESOLUTION
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Displacement parameters | Biso mean: 53 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.92 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.8 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.332 |