[English] 日本語
Yorodumi- PDB-1fr6: REFINED CRYSTAL STRUCTURE OF BETA-LACTAMASE FROM CITROBACTER FREU... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fr6 | ||||||
---|---|---|---|---|---|---|---|
Title | REFINED CRYSTAL STRUCTURE OF BETA-LACTAMASE FROM CITROBACTER FREUNDII INDICATES A MECHANISM FOR BETA-LACTAM HYDROLYSIS | ||||||
Components | BETA-LACTAMASE | ||||||
Keywords | HYDROLASE / Antibiotic Resistance / Class C Beta-Lactamase / Monobactum | ||||||
Function / homology | Function and homology information antibiotic catabolic process / beta-lactamase activity / beta-lactamase / outer membrane-bounded periplasmic space / response to antibiotic Similarity search - Function | ||||||
Biological species | Citrobacter freundii (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.5 Å | ||||||
Authors | Oefner, C. / D'Arcy, A. / Daly, J.J. / Winkler, F.K. | ||||||
Citation | Journal: Nature / Year: 1990 Title: Refined crystal structure of beta-lactamase from Citrobacter freundii indicates a mechanism for beta-lactam hydrolysis. Authors: Oefner, C. / D'Arcy, A. / Daly, J.J. / Gubernator, K. / Charnas, R.L. / Heinze, I. / Hubschwerlen, C. / Winkler, F.K. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1fr6.cif.gz | 154.8 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1fr6.ent.gz | 128.2 KB | Display | PDB format |
PDBx/mmJSON format | 1fr6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fr/1fr6 ftp://data.pdbj.org/pub/pdb/validation_reports/fr/1fr6 | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 39755.250 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Citrobacter freundii (bacteria) / Strain: 1203 / References: UniProt: Q46041, beta-lactamase #2: Chemical | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.46 % |
---|---|
Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 1.6M sodium-potassium phosphate , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 277 K |
---|---|
Diffraction source | Source: ROTATING ANODE / Type: ELLIOTT GX-21 / Wavelength: 1.5418 |
Detector | Type: XENTRONICS / Detector: AREA DETECTOR / Date: Jan 1, 1989 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→50 Å / Num. all: 27294 / Num. obs: 22927 / % possible obs: 84 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.09 |
-Processing
Software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.5→6 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: tnt /
| ||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→6 Å
| ||||||||||||
Refine LS restraints |
|