+Open data
-Basic information
Entry | Database: PDB / ID: 1fj7 | ||||||
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Title | SOLUTION STRUCTURE OF NUCLEOLIN RBD1 | ||||||
Components | NUCLEOLIN RBD1 | ||||||
Keywords | STRUCTURAL PROTEIN / RNP / RBD / RRM / RNA binding domain / Nucleolus | ||||||
Function / homology | Function and homology information positive regulation of mRNA splicing, via spliceosome / telomeric DNA binding / spliceosomal complex / ribonucleoprotein complex / nucleolus / RNA binding / cytoplasm Similarity search - Function | ||||||
Biological species | Mesocricetus auratus (golden hamster) | ||||||
Method | SOLUTION NMR / simulated annealing using X-PLOR 3.841 | ||||||
Authors | Allain, F.H.-T. / Gilbert, D.E. / Bouvet, P. / Feigon, J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: Solution structure of the two N-terminal RNA-binding domains of nucleolin and NMR study of the interaction with its RNA target. Authors: Allain, F.H. / Gilbert, D.E. / Bouvet, P. / Feigon, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fj7.cif.gz | 1018.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fj7.ent.gz | 858.6 KB | Display | PDB format |
PDBx/mmJSON format | 1fj7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fj7_validation.pdf.gz | 345.6 KB | Display | wwPDB validaton report |
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Full document | 1fj7_full_validation.pdf.gz | 716.7 KB | Display | |
Data in XML | 1fj7_validation.xml.gz | 124.3 KB | Display | |
Data in CIF | 1fj7_validation.cif.gz | 163.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fj/1fj7 ftp://data.pdbj.org/pub/pdb/validation_reports/fj/1fj7 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11029.398 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mesocricetus auratus (golden hamster) / Plasmid: BL21(DE3)PLYSS / Production host: Escherichia coli (E. coli) / References: UniProt: P08199 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
-Sample preparation
Details | Contents: 1mM U-15N,13C; 50mM potassium phosphate buffer; 100 mM KCl, 5mM deuterated DTT, pH 6.2 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 150 / pH: 6.2 / Pressure: ambient / Temperature: 293 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing using X-PLOR 3.841 / Software ordinal: 1 Details: structures are based on 1391 restraints, 1335 are NOE-derived and 56 are from hydrogen bonds | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 50 / Conformers submitted total number: 34 |