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- PDB-1fir: CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTION PRIMER TRNA(LYS3) -
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Open data
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Basic information
Entry | Database: PDB / ID: 1fir | ||||||
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Title | CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTION PRIMER TRNA(LYS3) | ||||||
![]() | HIV-1 REVERSE TRANSCRIPTION PRIMER TRNA(LYS3) | ||||||
![]() | RNA / mammalian transfert ribonucleic acid / HIV-1 primer tRNA / amino acid transport / canonical tRNA structure / canonical anticodon / frameshifting / codon/anticodon mimicry / modified bases | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Benas, P. / Dumas, P. | ||||||
![]() | ![]() Title: The crystal structure of HIV reverse-transcription primer tRNA(Lys,3) shows a canonical anticodon loop. Authors: Benas, P. / Bec, G. / Keith, G. / Marquet, R. / Ehresmann, C. / Ehresmann, B. / Dumas, P. #1: ![]() Title: Molecular blocs in tRNAs that could prevent +1 frameshifting Authors: Benas, P. / Dumas, P. #2: ![]() Title: Studies on the ternary initiation complex of HIV-1 reverse transcription and crystallographic structures of two partners Authors: Benas, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 53.5 KB | Display | ![]() |
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PDB format | ![]() | 42.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 416 KB | Display | ![]() |
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Full document | ![]() | 428.6 KB | Display | |
Data in XML | ![]() | 8 KB | Display | |
Data in CIF | ![]() | 9.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4tnaS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: RNA chain | Mass: 24847.088 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: THIS SEQUENCE OCCURS NATURALLY IN HUMANS, BOVINES AND CHICKENS Source: (natural) ![]() ![]() |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-NA / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.12 Å3/Da / Density % sol: 78.73 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 50 mM MES-KOH pH 5.6, 1.6 to 1.8 M Li2SO4, 10 mM MgCl2, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal | *PLUS Density % sol: 78.7 % | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 2, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9805 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→40 Å / Num. all: 6482 / Num. obs: 6482 / % possible obs: 88.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.2 % / Rsym value: 0.081 / Net I/σ(I): 10.5 |
Reflection shell | Resolution: 3.3→3.42 Å / Redundancy: 2.2 % / Mean I/σ(I) obs: 4.3 / Num. unique all: 503 / Rsym value: 0.165 / % possible all: 71.6 |
Reflection | *PLUS Rmerge(I) obs: 0.081 |
Reflection shell | *PLUS % possible obs: 71.6 % / Rmerge(I) obs: 0.165 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4TNA Resolution: 3.3→40 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 836049.77 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 3 Stereochemistry target values: modified CNS libraries (Parkinson et al.) Details: R and free R improved by 12% and 11% respectively by a proper mask definition (see primary ref.)
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 34.19 Å2 / ksol: 0.03 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.3→40 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.3→3.51 Å / Rfactor Rfree error: 0.04 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.4 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS σ(F): 3 / % reflection Rfree: 9.2 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 33.2 Å2 | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.234 / % reflection Rfree: 4.9 % / Rfactor Rwork: 0.221 |