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Open data
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Basic information
Entry | Database: PDB / ID: 1fhz | ||||||||||||||||||
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Title | PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION | ||||||||||||||||||
![]() | DNA (5'-D(*![]() DNA / psoralen / cross-linked DNA / Holliday junction / DNA-drug complex | Function / homology | 4'-HYDROXYMETHYL-4,5',8-TRIMETHYLPSORALEN / DNA | ![]() Method | ![]() ![]() ![]() ![]() Eichman, B.F. / Mooers, B.H.M. / Alberti, M. / Hearst, J.E. / Ho, P.S. | ![]() ![]() Title: The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions. Authors: Eichman, B.F. / Mooers, B.H. / Alberti, M. / Hearst, J.E. / Ho, P.S. #1: ![]() Title: The Holliday junction in an inverted repeat DNA sequence: Sequence effects on the structure of four-way junctions Authors: Eichman, B.F. / Vargason, J.M. / Mooers, B.H.M. / Ho, P.S. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 21.2 KB | Display | ![]() |
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PDB format | ![]() | 14.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 658.9 KB | Display | ![]() |
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Full document | ![]() | 660.4 KB | Display | |
Data in XML | ![]() | 3.8 KB | Display | |
Data in CIF | ![]() | 4.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1fhyC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | A four-way junction is a dimer constructed from chains A and B and a symmetry partner generated by the two-fold |
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Components
#1: DNA chain | Mass: 3045.992 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: DNA IS CROSS-LINKED BY PSORALEN #2: Chemical | ChemComp-PSO / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.89 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7 Details: sodium cacodylate, MgCl2, MPD, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K | ||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Oct 8, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→33.39 Å / Num. all: 2900 / Num. obs: 2900 / % possible obs: 96.7 % / Observed criterion σ(I): -3 / Redundancy: 3.3 % / Rmerge(I) obs: 0.067 / Net I/σ(I): 12.3 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.1 / Num. unique all: 284 / % possible all: 98.3 |
Reflection | *PLUS Lowest resolution: 33.4 Å / Num. measured all: 9596 |
Reflection shell | *PLUS % possible obs: 98.3 % / Mean I/σ(I) obs: 8.1 |
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Processing
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Refinement | Method to determine structure: ![]() Details: ANISOTROPIC B VALUES WERE APPLIED TO FCALC DURING REFINEMENT IN ORDER TO SCALE FCALC TO FOBS
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.2→33.39 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 33.4 Å / Rfactor Rfree: 0.284 / Rfactor Rwork: 0.222 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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