[English] 日本語
![](img/lk-miru.gif)
- PDB-1fhj: CRYSTAL STRUCTURE OF AQUOMET HEMOGLOBIN-I OF THE MANED WOLF (CHRY... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1fhj | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE OF AQUOMET HEMOGLOBIN-I OF THE MANED WOLF (CHRYSOCYON BRACHYURUS) AT 2.0 RESOLUTION. | ||||||
![]() |
| ||||||
![]() | OXYGEN STORAGE/TRANSPORT / maned wolf / hemoglobin / metahemoglobin / OXYGEN STORAGE-TRANSPORT COMPLEX | ||||||
Function / homology | ![]() hemoglobin alpha binding / haptoglobin binding / haptoglobin-hemoglobin complex / organic acid binding / hemoglobin complex / hydrogen peroxide catabolic process / oxygen carrier activity / oxygen binding / peroxidase activity / blood microparticle ...hemoglobin alpha binding / haptoglobin binding / haptoglobin-hemoglobin complex / organic acid binding / hemoglobin complex / hydrogen peroxide catabolic process / oxygen carrier activity / oxygen binding / peroxidase activity / blood microparticle / iron ion binding / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fadel, V. / de Azevedo, W.F. | ||||||
![]() | ![]() Title: Crystal structure of hemoglobin from the maned wolf (Chrysocyon brachyurus) using synchrotron radiation. Authors: Fadel, V. / Canduri, F. / Olivieri, J.R. / Smarra, A.L. / Colombo, M.F. / Bonilla-Rodriguez, G.O. / de Azevedo, W.F. #1: ![]() Title: Crystallization, Preliminary X-ray Diffraction Analysis and Patterson Search of Oxyhemoglobin I from the Wolf (Chrysocyon brachiurus) Authors: Smarra, A.L. / Fadel, V. / Dellamano, M. / Olivieri, J.R. / de Azevedo Jr., W.F. / Bonilla-Rodriguez, G.O. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 132.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 104.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.8 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.8 MB | Display | |
Data in XML | ![]() | 28.1 KB | Display | |
Data in CIF | ![]() | 38.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1hhoS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Details | The asymmetric unit has a complete tetramer formed by two alpha chains and two beta chains. |
-
Components
#1: Protein | Mass: 15270.329 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Protein | Mass: 16020.366 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #3: Chemical | ChemComp-HEM / #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.44 % |
---|---|
Crystal grow | Temperature: 295 K / Method: vapor diffusion / Details: PEG 1500 30%, VAPOR DIFFUSION, temperature 295K |
-Data collection
Diffraction | Mean temperature: 300 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 27, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.37 Å / Relative weight: 1 |
Reflection | Resolution: 1.864→72.548 Å / Num. all: 53941 / Num. obs: 50240 / % possible obs: 93.1 % / Observed criterion σ(I): 1 / Redundancy: 2.62 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 9.4 |
Reflection shell | Resolution: 1.86→1.9 Å / Redundancy: 2.38 % / Rmerge(I) obs: 0.583 / Num. unique all: 2799 / % possible all: 78.7 |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: HUMAN OXYHEMOGLOBIN pdb id 1HHO Resolution: 1.8→10 Å / σ(F): 2 / σ(I): 0 Details: Refmac was also used for refinement. THE SIMULLATED ANNEALING METHOD (X-PLOR) AND MAXIMUM LIKELIHOOD METHOD (REFMAC) WAS EMPLOYED. Data was used to 1.8 angstroms, but with low completeness.
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
| |||||||||||||||||||||||||
Refine LS restraints |
|