+Open data
-Basic information
Entry | Database: PDB / ID: 1fgb | ||||||
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Title | TOXIN | ||||||
Components | CHOLERA TOXIN B SUBUNIT PENTAMER | ||||||
Keywords | ENTEROTOXIN / CHOLERA TOXIN / CHOLERAGENOID / ADP-RIBOSYLATION | ||||||
Function / homology | Function and homology information host cell surface binding / galactose binding / catalytic complex / positive regulation of tyrosine phosphorylation of STAT protein / toxin activity / periplasmic space / host cell plasma membrane / extracellular region / membrane Similarity search - Function | ||||||
Biological species | Vibrio cholerae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.4 Å | ||||||
Authors | Zhang, R.-G. / Westbrook, E. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1995 Title: The 2.4 A crystal structure of cholera toxin B subunit pentamer: choleragenoid. Authors: Zhang, R.G. / Westbrook, M.L. / Westbrook, E.M. / Scott, D.L. / Otwinowski, Z. / Maulik, P.R. / Reed, R.A. / Shipley, G.G. #1: Journal: J.Mol.Biol. / Year: 1995 Title: The Three-Dimensional Crystal Structure of Cholera Toxin Authors: Zhang, R.G. / Scott, D.L. / Westbrook, M.L. / Nance, S. / Spangler, B.D. / Shipley, G.G. / Westbrook, E.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fgb.cif.gz | 115.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fgb.ent.gz | 89.3 KB | Display | PDB format |
PDBx/mmJSON format | 1fgb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fgb_validation.pdf.gz | 400.9 KB | Display | wwPDB validaton report |
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Full document | 1fgb_full_validation.pdf.gz | 463.2 KB | Display | |
Data in XML | 1fgb_validation.xml.gz | 20.5 KB | Display | |
Data in CIF | 1fgb_validation.cif.gz | 30.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fg/1fgb ftp://data.pdbj.org/pub/pdb/validation_reports/fg/1fgb | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 11623.267 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Vibrio cholerae (bacteria) / Strain: 569B / References: UniProt: P01556 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.42 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.75 / Method: vapor diffusion, hanging drop / Details: Maulik, P.R., (1988) J. Biol. Chem., 263, 9499. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Aug 15, 1989 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Rmerge(I) obs: 0.048 |
Reflection | *PLUS Highest resolution: 2.38 Å / Num. obs: 16703 / % possible obs: 85 % / Num. measured all: 27472 / Rmerge(I) obs: 0.0294 |
-Processing
Software |
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Refinement | Highest resolution: 2.4 Å / σ(F): 1.5 /
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Displacement parameters | Biso mean: 20.37 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.4 Å
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Refine LS restraints |
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Software | *PLUS Name: PROFFT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.171 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |