+Open data
-Basic information
Entry | Database: PDB / ID: 1fe4 | |||||||||
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Title | CRYSTAL STRUCTURE OF MERCURY-HAH1 | |||||||||
Components | COPPER TRANSPORT PROTEIN ATOX1 | |||||||||
Keywords | METAL TRANSPORT / beta-alpha-beta-beta-alpha-beta | |||||||||
Function / homology | Function and homology information metallochaperone activity / Ion influx/efflux at host-pathogen interface / copper-dependent protein binding / copper chaperone activity / copper ion transport / Detoxification of Reactive Oxygen Species / cuprous ion binding / intracellular copper ion homeostasis / response to oxidative stress / copper ion binding / cytosol Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.75 Å | |||||||||
Authors | Wernimont, A.K. / Huffman, D.L. / Lamb, A.L. / O'Halloran, T.V. / Rosenzweig, A.C. | |||||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: Structural basis for copper transfer by the metallochaperone for the Menkes/Wilson disease proteins. Authors: Wernimont, A.K. / Huffman, D.L. / Lamb, A.L. / O'Halloran, T.V. / Rosenzweig, A.C. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fe4.cif.gz | 41.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fe4.ent.gz | 28.3 KB | Display | PDB format |
PDBx/mmJSON format | 1fe4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fe/1fe4 ftp://data.pdbj.org/pub/pdb/validation_reports/fe/1fe4 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein / Sugars , 2 types, 3 molecules AB
#1: Protein | Mass: 7412.646 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Organ: LIVER / Plasmid: PMAGDA / Production host: Escherichia coli (E. coli) / References: UniProt: O00244 #2: Polysaccharide | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose / sucrose / | |
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-Non-polymers , 4 types, 126 molecules
#3: Chemical | ChemComp-IUM / | ||||
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#4: Chemical | #5: Chemical | ChemComp-HG / | #6: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.46 Å3/Da / Density % sol: 64.45 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: MES buffer, ammonium sulfate, magnesium chloride, dithiothreitol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 5ID-B / Wavelength: 1.02 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jun 7, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.02 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→30 Å / Num. all: 288469 / Num. obs: 20119 / % possible obs: 97.8 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 7 % / Biso Wilson estimate: 25.2 Å2 / Rmerge(I) obs: 0.075 / Net I/σ(I): 6.6 |
Reflection shell | Resolution: 1.75→1.81 Å / Redundancy: 3 % / Rmerge(I) obs: 0.37 / % possible all: 87.2 |
Reflection | *PLUS % possible obs: 98.4 % / Num. measured all: 288469 |
Reflection shell | *PLUS % possible obs: 87.2 % / Rmerge(I) obs: 0.37 / Mean I/σ(I) obs: 1.5 |
-Processing
Software |
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Refinement | Resolution: 1.75→30 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.75→30 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 30 Å / σ(F): 0 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 31 Å2 | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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