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Open data
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Basic information
Entry | Database: PDB / ID: 1f8x | ||||||
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Title | CRYSTAL STRUCTURE OF NUCLEOSIDE 2-DEOXYRIBOSYLTRANSFERASE | ||||||
![]() | NUCLEOSIDE 2-DEOXYRIBOSYLTRANSFERASE | ||||||
![]() | TRANSFERASE / active site / alpha/beta protein / biocatalyst / nucleoside | ||||||
Function / homology | ![]() nucleoside deoxyribosyltransferase / nucleotide salvage / nucleoside deoxyribosyltransferase activity Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Armstrong, S.R. / Cook, W.J. / Short, S.A. / Ealick, S.E. | ||||||
![]() | ![]() Title: Crystal structures of nucleoside 2-deoxyribosyltransferase in native and ligand-bound forms reveal architecture of the active site. Authors: Armstrong, S.R. / Cook, W.J. / Short, S.A. / Ealick, S.E. #1: ![]() Title: Crystallization and Preliminary X-ray Investigation of Recombinant Lactobacillus leichmannii Nucleoside Deoxyribosyltransferase Authors: Cook, W.J. / Short, S.A. / Ealick, S.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 73.8 KB | Display | ![]() |
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PDB format | ![]() | 56.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18098.354 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: Q9R5V5, nucleoside deoxyribosyltransferase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.96 Å3/Da / Density % sol: 68.95 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 5.4 Details: ammonium sulfate, citrate buffer, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction |
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Detector |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 | ||||||||||||||||
Reflection | Resolution: 2.5→100 Å / Num. all: 223078 / Num. obs: 19875 / % possible obs: 95 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 11.2 % / Rmerge(I) obs: 0.115 |
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Processing
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Refinement | Resolution: 2.5→5 Å / σ(F): 2 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.5→5 Å
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Refine LS restraints |
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