+Open data
-Basic information
Entry | Database: PDB / ID: 1f2j | ||||||
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Title | CRYSTAL STRUCTURE ANALYSIS OF ALDOLASE FROM T. BRUCEI | ||||||
Components | FRUCTOSE-BISPHOSPHATE ALDOLASE, GLYCOSOMAL | ||||||
Keywords | LYASE / BETA BARREL / PTS SIGNAL / ALDOLASE / TRYPANOSOMA / FRUCTOSE 1 / 6 BISPHOSPHATE | ||||||
Function / homology | Function and homology information fructose-bisphosphate aldolase / fructose-bisphosphate aldolase activity / glycosome / glycolytic process Similarity search - Function | ||||||
Biological species | Trypanosoma brucei (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Chudzik, D.M. / Michels, P.A. / De Walque, S. / Hol, W.G.J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: Structures of type 2 peroxisomal targeting signals in two trypanosomatid aldolases. Authors: Chudzik, D.M. / Michels, P.A. / de Walque, S. / Hol, W.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f2j.cif.gz | 88.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f2j.ent.gz | 66 KB | Display | PDB format |
PDBx/mmJSON format | 1f2j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f2/1f2j ftp://data.pdbj.org/pub/pdb/validation_reports/f2/1f2j | HTTPS FTP |
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-Related structure data
Related structure data | 1epxC 1adoS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 40939.988 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma brucei (eukaryote) / Plasmid: PET3A / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 PLYSS / References: UniProt: P07752, fructose-bisphosphate aldolase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.7 Å3/Da / Density % sol: 73.76 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 4.8 Details: 100 mM sodium acetate pH 4.9, 1.9 M ammonium sulfate, 20 mM L-cysteine, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 150 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-1 / Wavelength: 0.98 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 7, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→84.5 Å / Num. all: 29298 / Num. obs: 479727 / % possible obs: 99.9 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 6.3 % / Biso Wilson estimate: 24.2 Å2 / Rmerge(I) obs: 0.092 / Net I/σ(I): 21.3 |
Reflection shell | Resolution: 1.9→2.1 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.121 / Num. unique all: 5143 / % possible all: 72.1 |
Reflection | *PLUS Num. obs: 29298 / Num. measured all: 479727 |
Reflection shell | *PLUS % possible obs: 72.1 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: TETRAMERIC RABBIT ALDOLASE (1ADO) Resolution: 1.9→15 Å / σ(F): 10000000 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.9→15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→2 Å / Total num. of bins used: 8
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |