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Yorodumi- PDB-1f04: SOLUTION STRUCTURE OF OXIDIZED BOVINE MICROSOMAL CYTOCHROME B5 MU... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f04 | ||||||
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Title | SOLUTION STRUCTURE OF OXIDIZED BOVINE MICROSOMAL CYTOCHROME B5 MUTANT (E44A, E48A, E56A, D60A) AND ITS INTERACTION WITH CYTOCHROME C | ||||||
Components | CYTOCHROME B5 | ||||||
Keywords | ELECTRON TRANSPORT / CYTOCHROME B5 / PROTEIN RECOGNITION / SOLUTION STRUCTURE / PARAMAGNETIC NMR | ||||||
Function / homology | Function and homology information Vitamin C (ascorbate) metabolism / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / mitochondrial outer membrane / intracellular membrane-bounded organelle / heme binding / endoplasmic reticulum membrane / metal ion binding Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | SOLUTION NMR / TORSION ANGLE MOLECULAR | ||||||
Authors | Wu, Y.B. / Lu, J. / Qian, C.M. / Tang, W.X. / Li, E.C. / Wang, J.F. / Wang, Y.H. / Wang, W.H. / Lu, J.X. / Xie, Y. / Huang, Z.X. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 2001 Title: Solution structure of cytochrome b(5) mutant (E44/48/56A/D60A) and its interaction with cytochrome c. Authors: Wu, Y. / Wang, Y. / Qian, C. / Lu, J. / Li, E. / Wang, W. / Lu, J. / Xie, Y. / Wang, J. / Zhu, D. / Huang, Z. / Tang, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f04.cif.gz | 920.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f04.ent.gz | 769.6 KB | Display | PDB format |
PDBx/mmJSON format | 1f04.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1f04_validation.pdf.gz | 463.6 KB | Display | wwPDB validaton report |
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Full document | 1f04_full_validation.pdf.gz | 664.8 KB | Display | |
Data in XML | 1f04_validation.xml.gz | 45.1 KB | Display | |
Data in CIF | 1f04_validation.cif.gz | 75 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f0/1f04 ftp://data.pdbj.org/pub/pdb/validation_reports/f0/1f04 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9257.262 Da / Num. of mol.: 1 / Mutation: E44A, E48A, E56A, D60A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Plasmid: PUC 19 / Production host: Escherichia coli (E. coli) / Strain (production host): JM83 / References: UniProt: P00171 |
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#2: Chemical | ChemComp-HEM / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 10 mM / pH: 7 / Pressure: 1 atm / Temperature: 303 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: TORSION ANGLE MOLECULAR / Software ordinal: 1 Details: DYNAMICS SIMULATED ANNEALING, RESTRAINED ENERGY MINIMIZATION; PSEUDOCONTACT SHIFTS WERE USED IN THE CALCULATION AND IN THE MINIMIZATION AS FURTHER NON-CLASSICAL CONSTRAINTS | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 35 / Conformers submitted total number: 35 |