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- PDB-1esy: NMR STRUCTURE OF STEM LOOP SL2 OF THE HIV-1 PSI RNA PACKAGING SIG... -
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Basic information
Entry | Database: PDB / ID: 1esy | ||||||||||||||||||
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Title | NMR STRUCTURE OF STEM LOOP SL2 OF THE HIV-1 PSI RNA PACKAGING SIGNAL REVEALS A NOVEL A-U-A BASE-TRIPLE PLATFORM | ||||||||||||||||||
![]() | RNA (5'-R(P*![]() RNA / HIV-1 / Splice-donor site / platform motif | Function / homology | RNA / RNA (> 10) | ![]() Method | SOLUTION NMR / Distance Geometry, Simulated annealing, Energy mimimization | ![]() Amarasinghe, G.K. / De Guzman, R.N. / Turner, R.B. / Summers, M.F. | ![]() ![]() Title: NMR structure of stem-loop SL2 of the HIV-1 psi RNA packaging signal reveals a novel A-U-A base-triple platform. Authors: Amarasinghe, G.K. / De Guzman, R.N. / Turner, R.B. / Summers, M.F. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 253.2 KB | Display | ![]() |
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PDB format | ![]() | 202.2 KB | Display | ![]() |
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-Validation report
Summary document | ![]() | 328 KB | Display | ![]() |
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Full document | ![]() | 432.1 KB | Display | |
Data in XML | ![]() | 24.9 KB | Display | |
Data in CIF | ![]() | 38.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 6148.712 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: T7 Transcript |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: experimental temperature set to either 298 or 278 |
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Sample preparation
Details |
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Sample conditions | Ionic strength: 25 mM NaCl / pH: 5.5 / Pressure: ambient / Temperature: 298 K | ||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: Distance Geometry, Simulated annealing, Energy mimimization Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |