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- PDB-1eno: BRASSICA NAPUS ENOYL ACP REDUCTASE/NAD BINARY COMPLEX AT PH 8.0 A... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1eno | ||||||
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Title | BRASSICA NAPUS ENOYL ACP REDUCTASE/NAD BINARY COMPLEX AT PH 8.0 AND ROOM TEMPERATURE | ||||||
![]() | ENOYL ACYL CARRIER PROTEIN REDUCTASE | ||||||
![]() | OXIDOREDUCTASE / PLANT LIPID BIOSYNTHESIS | ||||||
Function / homology | ![]() enoyl-[acyl-carrier-protein] reductase (NADH) / enoyl-[acyl-carrier-protein] reductase (NADH) activity / chloroplast / fatty acid biosynthetic process Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Rafferty, J.B. / Rice, D.W. | ||||||
![]() | ![]() Title: Common themes in redox chemistry emerge from the X-ray structure of oilseed rape (Brassica napus) enoyl acyl carrier protein reductase. Authors: Rafferty, J.B. / Simon, J.W. / Baldock, C. / Artymiuk, P.J. / Baker, P.J. / Stuitje, A.R. / Slabas, A.R. / Rice, D.W. #1: ![]() Title: Crystallization of the Nadh-Specific Enoyl Acyl Carrier Protein Reductase from Brassica Napus Authors: Rafferty, J.B. / Simon, J.W. / Stuitje, A.R. / Slabas, A.R. / Fawcett, T. / Rice, D.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.2 KB | Display | ![]() |
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PDB format | ![]() | 52.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 472 KB | Display | ![]() |
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Full document | ![]() | 477.5 KB | Display | |
Data in XML | ![]() | 8.7 KB | Display | |
Data in CIF | ![]() | 13.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32977.156 Da / Num. of mol.: 1 / Mutation: S1A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P80030, enoyl-[acyl-carrier-protein] reductase (NADH) |
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#2: Chemical | ChemComp-NAD / |
#3: Water | ChemComp-HOH / |
Nonpolymer details | THE ELECTRON DENSITY FOR THE NICOTINAMIDE RING AND ASSOCIATED RIBOSE SUGAR OF THE NAD COFACTOR WAS ...THE ELECTRON DENSITY FOR THE NICOTINAMI |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 41 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8 / Details: pH 8.0 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 17 ℃ / pH: 6.2 / Method: vapor diffusion, hanging drop / Details: Rafferty, J.B., (1994) J.Mol.Biol., 237, 240. | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() |
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Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Oct 15, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 20642 / % possible obs: 83 % / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Rmerge(I) obs: 0.047 |
Reflection | *PLUS Highest resolution: 1.9 Å / Num. measured all: 93536 |
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Processing
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Refinement | Resolution: 1.9→10 Å / Num. reflection obs: 20557 / σ(F): 0 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.164 / Rfactor Rwork: 0.164 | ||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: t_plane_restr / Dev ideal: 0.02 / Weight: 5 |