+Open data
-Basic information
Entry | Database: PDB / ID: 1eiz | ||||||
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Title | FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE | ||||||
Components | FTSJ | ||||||
Keywords | TRANSFERASE / ftsj / methyltransferase / adomet / adenosyl methionine / heat shock proteinS / 23S RIBOSOMAL RNA | ||||||
Function / homology | Function and homology information 23S rRNA (uridine2552-2'-O)-methyltransferase / rRNA (uridine-2'-O-)-methyltransferase activity / RNA methylation / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 1.7 Å | ||||||
Authors | Bugl, H. / Fauman, E.B. / Staker, B.L. / Zheng, F. / Kushner, S.R. / Saper, M.A. / Bardwell, J.C.A. / Jakob, U. | ||||||
Citation | Journal: Mol.Cell / Year: 2000 Title: RNA methylation under heat shock control. Authors: Bugl, H. / Fauman, E.B. / Staker, B.L. / Zheng, F. / Kushner, S.R. / Saper, M.A. / Bardwell, J.C. / Jakob, U. #1: Journal: J.Biol.Chem. / Year: 2000 Title: The FtsJ/RrmJ heat shock protein of escherichia coli is a 23 S ribosomal RNA methyltransferase. Authors: Caldas, T. / Binet, E. / Bouloc, P. / Costa, A. / Desgres, J. / Richarme, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eiz.cif.gz | 52.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eiz.ent.gz | 36.5 KB | Display | PDB format |
PDBx/mmJSON format | 1eiz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ei/1eiz ftp://data.pdbj.org/pub/pdb/validation_reports/ei/1eiz | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | biological assembly is a monomer in solution |
-Components
#1: Protein | Mass: 19937.207 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: PET11A / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) References: UniProt: P28692, UniProt: P0C0R7*PLUS, Transferases; Transferring one-carbon groups; Methyltransferases |
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#2: Chemical | ChemComp-SAM / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.92 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.65 Details: 1mM AdoMet, 33% PEG 4000, 0.19 M ammonium acetate, 0.1 M sodium citrate pH 5.65, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.7 / Method: vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 160 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Feb 2, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→20 Å / Num. all: 29034 / Num. obs: 24364 / % possible obs: 94 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7 % / Biso Wilson estimate: 17.6 Å2 / Rmerge(I) obs: 0.058 / Net I/σ(I): 14 |
Reflection shell | Resolution: 1.5→1.53 Å / Redundancy: 4 % / Rmerge(I) obs: 0.371 / Num. unique all: 1195 / % possible all: 62.1 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 1.7→18.22 Å / Rfactor Rfree error: 0.007 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber / Details: bulk solvent correction
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 50.4512 Å2 / ksol: 0.394983 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.7→18.22 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.7→1.76 Å / Rfactor Rfree error: 0.038 / Total num. of bins used: 10
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.7 Å / σ(F): 0 / % reflection Rfree: 5.4 % / Rfactor obs: 0.191 / Rfactor Rwork: 0.19 | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 18 Å2 | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.389 / % reflection Rfree: 5.9 % / Rfactor Rwork: 0.369 |