The structure comprises two hevein-like domains, each homologous to those of Wheat Germ Agglutinin ...The structure comprises two hevein-like domains, each homologous to those of Wheat Germ Agglutinin (PDB code: 9wga). Each domain contains a saccharide-binding site. The N-terminal residue is pyrrolidone carboxylic acid (PCA). A dual conformation is seen at Gly79, Gly80. No electron density is seen for the side chain of Arg33 beyond the CB atom. No interpretable density is seen for C-terminal residues Ser87, Ser88, and Ser89.
構造決定の手法: 多重同系置換 / 解像度: 1.66→18 Å / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: Engh & Huber 詳細: The structure was determined to 2.45A resolution by MIR methods based on 4 Hg derivatives (rotating-anode X-ray source) with solvent flattening, and refined to 1.66A resolution with ...詳細: The structure was determined to 2.45A resolution by MIR methods based on 4 Hg derivatives (rotating-anode X-ray source) with solvent flattening, and refined to 1.66A resolution with synchrotron data using a bulk solvent correction and anisotropic scale factor.