[English] 日本語
![](img/lk-miru.gif)
- PDB-1efz: MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRN... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1efz | ||||||
---|---|---|---|---|---|---|---|
Title | MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRNA-GUANINE TRANSGLYCOSYLASE TO ELUCIDATE THE ROLE OF SERINE 103 FOR ENZYMATIC ACTIVITY | ||||||
![]() | TRNA-GUANINE TRANSGLYCOSYLASE | ||||||
![]() | TRANSFERASE / TRNA-MODIFYING ENZYME / GLYCOSYLTRANSFERASE | ||||||
Function / homology | ![]() tRNA-guanosine34 preQ1 transglycosylase / tRNA wobble guanine modification / tRNA-guanosine(34) queuine transglycosylase activity / tRNA-guanine transglycosylation / queuosine biosynthetic process / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Gradler, U. / Ficner, R. / Garcia, G.A. / Stubbs, M.T. / Klebe, G. / Reuter, K. | ||||||
![]() | ![]() Title: Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity. Authors: Gradler, U. / Ficner, R. / Garcia, G.A. / Stubbs, M.T. / Klebe, G. / Reuter, K. #1: ![]() Title: Crystal Structure of tRNA-Guanine Transglycosylase: RNA Modification by Base Exchange. Authors: Romier, C. / Reuter, K. / Suck, D. / Ficner, R. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 93.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 69.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 42909.703 Da / Num. of mol.: 1 / Mutation: S103A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P28720, tRNA-guanosine34 preQ1 transglycosylase |
---|---|
#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-PRF / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 49.03 % | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 8000, tris, dtt, dmso, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 25 ℃ | ||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: May 27, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2→71 Å / Num. obs: 27018 / Observed criterion σ(F): 2 / Observed criterion σ(I): 0.001 / Rmerge(I) obs: 0.057 |
Reflection | *PLUS % possible obs: 97.4 % |
-
Processing
Software |
| ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2→71 Å / σ(F): 2 / σ(I): 0.001
| ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→71 Å
| ||||||||||||||||||
Refine LS restraints |
|