+Open data
-Basic information
Entry | Database: PDB / ID: 1ecz | ||||||
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Title | PROTEASE INHIBITOR ECOTIN | ||||||
Components | ECOTIN | ||||||
Keywords | PROTEASE INHIBITOR / BETA-SHEET STRUCTURE / SERINE PROTEASE INHIBITOR / PERIPLASMIC | ||||||
Function / homology | Function and homology information serine-type endopeptidase inhibitor activity / outer membrane-bounded periplasmic space / protein homodimerization activity Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.68 Å | ||||||
Authors | Shin, D.H. / Suh, S.W. | ||||||
Citation | Journal: Protein Sci. / Year: 1996 Title: Crystal structure analyses of uncomplexed ecotin in two crystal forms: implications for its function and stability. Authors: Shin, D.H. / Song, H.K. / Seong, I.S. / Lee, C.S. / Chung, C.H. / Suh, S.W. #1: Journal: J.Mol.Biol. / Year: 1993 Title: Crystallization and Preliminary X-Ray Crystallographic Analysis of the Protease Inhibitor Ecotin Authors: Shin, D.H. / Hwang, K.Y. / Kim, K.K. / Lee, H.R. / Lee, C.S. / Chung, C.H. / Suh, S.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ecz.cif.gz | 80.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ecz.ent.gz | 62.6 KB | Display | PDB format |
PDBx/mmJSON format | 1ecz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ecz_validation.pdf.gz | 3.7 MB | Display | wwPDB validaton report |
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Full document | 1ecz_full_validation.pdf.gz | 3.7 MB | Display | |
Data in XML | 1ecz_validation.xml.gz | 20 KB | Display | |
Data in CIF | 1ecz_validation.cif.gz | 25.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ec/1ecz ftp://data.pdbj.org/pub/pdb/validation_reports/ec/1ecz | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.986319, -0.012247, 0.16439), Vector: |
-Components
#1: Protein | Mass: 16120.507 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: ESCHERICHIA COLI PROTEASE INHIBITOR / Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: PBS / Gene (production host): ETI GENE / Production host: Escherichia coli (E. coli) / References: UniProt: P23827 #2: Sugar | ChemComp-BOG / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.8 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 24.5-25.5 K / pH: 8.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH2R / Wavelength: 1.5418 |
Detector | Type: ENRAF-NONIUS FAST / Detector: DIFFRACTOMETER / Date: May 14, 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.68→30.7 Å / Num. obs: 7810 / % possible obs: 80 % / Observed criterion σ(I): 0.5 / Redundancy: 3.5 % / Rmerge(I) obs: 0.076 |
Reflection shell | Resolution: 2.68→2.8 Å / % possible all: 25.2 |
Reflection shell | *PLUS % possible obs: 25.2 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: ECOTIN PART OF ECOTIN-TRYPSIN COMPLEX Resolution: 2.68→6 Å / σ(F): 2
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Displacement parameters | Biso mean: 38 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.68→6 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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