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- PDB-1e7n: The N-terminal domain of beta-B2-crystallin resembles the putativ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1e7n | ||||||
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Title | The N-terminal domain of beta-B2-crystallin resembles the putative ancestral homodimer | ||||||
![]() | BETA-CRYSTALLIN B2 | ||||||
![]() | STRUCTURAL PROTEIN / EYE LENS PROTEIN / DOMAIN INTERACTIONS / 2-FOLD SYMMETRY | ||||||
Function / homology | ![]() structural constituent of eye lens / camera-type eye development / lens development in camera-type eye / visual perception / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Clout, N.J. / Basak, A. / Wieligmann, K. / Bateman, O.A. / Jaenicke, R. / Slingsby, C. | ||||||
![]() | ![]() Title: The N-Terminal Domain of Betab2-Crystallin Resembles the Putative Ancestral Homodimer. Authors: Clout, N.J. / Basak, A. / Wieligmann, K. / Bateman, O.A. / Jaenicke, R. / Slingsby, C. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 47.1 KB | Display | ![]() |
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PDB format | ![]() | 33.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 372 KB | Display | ![]() |
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Full document | ![]() | 375.5 KB | Display | |
Data in XML | ![]() | 5.3 KB | Display | |
Data in CIF | ![]() | 7.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.178, 0.984, -0.004), Vector: |
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Components
#1: Protein | Mass: 11911.123 Da / Num. of mol.: 2 / Fragment: N-TERMINAL DOMAIN, RESIDUES 2-107 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | Compound details | DOMINANT COMPONENT OF EYE LENS IN VERTEBRATE | Sequence details | ONLY N-TERMINAL DOMAIN PRESENT. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 45 % | |||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.50 | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging dropDetails: drop consists of equal amounts of protein and reservoir solutions | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→20 Å / Num. obs: 9079 / % possible obs: 99.8 % / Redundancy: 2.7 % / Biso Wilson estimate: 16.2 Å2 / Rmerge(I) obs: 0.057 |
Reflection | *PLUS Rmerge(I) obs: 0.05 |
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Processing
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Refinement | Method to determine structure: ![]() Details: THE N-TERMINAL 13 RESIDUE EXTENSION WAS NOT SEEN IN THE DENSITY C-TERMINAL LAST FOUR RESIDUES WERE NOT SEEN IN THE DENSITY MAPS
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Displacement parameters | Biso mean: 21.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.35→19.89 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.35→2.5 Å / Rfactor Rfree error: 0.034 / Total num. of bins used: 6
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rwork: 0.2049 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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