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Yorodumi- PDB-1e5g: Solution structure of central CP module pair of a pox virus compl... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1e5g | ||||||
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Title | Solution structure of central CP module pair of a pox virus complement inhibitor | ||||||
Components | COMPLEMENT CONTROL PROTEIN C3 | ||||||
Keywords | COMPLEMENT INHIBITOR | ||||||
Function / homology | Function and homology information : / complement binding / membrane => GO:0016020 / host cell plasma membrane / virion membrane / extracellular region Similarity search - Function | ||||||
Biological species | VACCINIA VIRUS | ||||||
Method | SOLUTION NMR / ARIA | ||||||
Authors | Henderson, C.E. / Bromek, K. / Mullin, N.P. / Smith, B.O. / Uhrin, D. / Barlow, P.N. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2001 Title: Solution Structure and Dynamics of the Central Ccp Module Pair of a Poxvirus Complement Control Protein Authors: Henderson, C.E. / Bromek, K. / Mullin, N.P. / Smith, B.O. / Uhrin, D. / Barlow, P.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1e5g.cif.gz | 1.6 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1e5g.ent.gz | 1.4 MB | Display | PDB format |
PDBx/mmJSON format | 1e5g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1e5g_validation.pdf.gz | 346.9 KB | Display | wwPDB validaton report |
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Full document | 1e5g_full_validation.pdf.gz | 781.9 KB | Display | |
Data in XML | 1e5g_validation.xml.gz | 146.2 KB | Display | |
Data in CIF | 1e5g_validation.cif.gz | 189.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e5/1e5g ftp://data.pdbj.org/pub/pdb/validation_reports/e5/1e5g | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12783.905 Da / Num. of mol.: 1 / Fragment: MODULES 2 AND 3 RESIDUES 84-203 Source method: isolated from a genetically manipulated source Source: (gene. exp.) VACCINIA VIRUS / Strain: COPENHAGEN / Gene: C21L / Plasmid: PPICZALPHAA WITHOUT HISTIDINE DEFICIENCY / Production host: PICHIA PASTORIS (fungus) / Strain (production host): KM71 / References: UniProt: P68639 |
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Compound details | FUNCTION: SERVES TO PROTECT THE VIRUS AGAINST COMPLEMENT ATTACK BY INHIBITING BOTH CLASSICAL AND ...FUNCTION: SERVES TO PROTECT THE VIRUS AGAINST COMPLEMENT |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: 15N-LABELLED VCP MODULES 2 AND 3 WAS USED TO OBTAIN HSQC NOESY AND TOCSY EXPERIMENTS TO ASSIGN THE BACKBONE ATOMS. 2D EXPERIMENTS WERE THEN USED IN CONJUNCTION WITH THE 3D TECHNIQUES TO ASSIGN ...Text: 15N-LABELLED VCP MODULES 2 AND 3 WAS USED TO OBTAIN HSQC NOESY AND TOCSY EXPERIMENTS TO ASSIGN THE BACKBONE ATOMS. 2D EXPERIMENTS WERE THEN USED IN CONJUNCTION WITH THE 3D TECHNIQUES TO ASSIGN THE SIDE CHAIN ATOMS AND THE STRUCTURE WAS CALCULATED USING UNAMBIGUOUS AND AMBIGUOUS RESTRAINTS |
-Sample preparation
Sample conditions | pH: 6.0 / Temperature: 310 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: ARIA / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION | ||||||||||||
NMR ensemble | Conformers submitted total number: 50 |