+Open data
-Basic information
Entry | Database: PDB / ID: 1e0e | ||||||||||||
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Title | N-terminal zinc-binding HHCC domain of HIV-2 integrase | ||||||||||||
Components | HUMAN IMMUNODEFICIENCY VIRUS TYPE 2 INTEGRASE | ||||||||||||
Keywords | INTEGRASE / AIDS / POLYPROTEIN / DIMER / ZINC-BINDING PROTEIN / HELIX-TURN-HELIX MOTIF | ||||||||||||
Function / homology | Function and homology information HIV-2 retropepsin / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / viral penetration into host nucleus ...HIV-2 retropepsin / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / viral penetration into host nucleus / RNA stem-loop binding / symbiont-mediated suppression of host gene expression / RNA-directed DNA polymerase activity / host cell / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / Hydrolases; Acting on ester bonds / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / viral translational frameshifting / symbiont entry into host cell / lipid binding / host cell nucleus / host cell plasma membrane / virion membrane / structural molecule activity / proteolysis / DNA binding / zinc ion binding / membrane Similarity search - Function | ||||||||||||
Biological species | HUMAN IMMUNODEFICIENCY VIRUS TYPE 2 | ||||||||||||
Method | SOLUTION NMR / simulated annealing | ||||||||||||
Authors | Eijkelenboom, A.P.A.M. / Van Den ent, F.M.I. / Plasterk, R.H.A. / Kaptein, R. / Boelens, R. | ||||||||||||
Citation | Journal: J.Biomol.NMR / Year: 2000 Title: Refined Solution Structure of the Dimeric N-Terminal Hhcc Domain of HIV-2 Integrase Authors: Eijkelenboom, A.P.A.M. / Van Den Ent, F.M.I. / Wechselberger, R. / Plasterk, R.H.A. / Kaptein, R. / Boelens, R. #1: Journal: Curr.Biol. / Year: 1997 Title: Solution Structure of the N-Terminal Hhcc Domain of HIV-2 Integrase: A Three-Helix Bundle Stabilized by Zinc Authors: Eijkelenboom, A.P.A.M. / Van Den Ent, F.M.I. / Vos, A. / Doreleijers, J.F. / Hard, K. / Tullius, T.D. / Plasterk, R.H.A. / Kaptein, R. / Boelens, R. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1e0e.cif.gz | 927.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1e0e.ent.gz | 769.6 KB | Display | PDB format |
PDBx/mmJSON format | 1e0e.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1e0e_validation.pdf.gz | 430.9 KB | Display | wwPDB validaton report |
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Full document | 1e0e_full_validation.pdf.gz | 663.8 KB | Display | |
Data in XML | 1e0e_validation.xml.gz | 42.8 KB | Display | |
Data in CIF | 1e0e_validation.cif.gz | 74.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e0/1e0e ftp://data.pdbj.org/pub/pdb/validation_reports/e0/1e0e | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6266.040 Da / Num. of mol.: 2 / Fragment: N-TERMINAL HHCC DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) HUMAN IMMUNODEFICIENCY VIRUS TYPE 2 (ISOLATE ROD) Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P04584 #2: Chemical | Sequence details | TER LYS: RESIDUES 47-55 ARE DISORDERED | |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR details | Text: DETAILS CAN BE FOUND IN THE JRNL CITATION |
-Sample preparation
Details | Contents: D2O AND 5% D2O/95% H2O |
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Sample conditions | pH: 6.5 / Temperature: 300 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: LOW OVERALL ENERGY / Conformers calculated total number: 50 / Conformers submitted total number: 32 |