Mass: 8076.179 Da / Num. of mol.: 2 / Fragment: SHADOW CHROMO DOMAIN, RESIDUES 104-171 Source method: isolated from a genetically manipulated source Source: (gene. exp.) MUS MUSCULUS (house mouse) / Plasmid: PET16B / Production host: Escherichia coli BL21(DE3) / References: UniProt: P83917
Sequence details
HIS A 102 CLONING ARTIFACT MUTATION ARISING FROM VECTOR MET A 103 CLONING ARTIFACT MUTATION ARISING ...HIS A 102 CLONING ARTIFACT MUTATION ARISING FROM VECTOR MET A 103 CLONING ARTIFACT MUTATION ARISING FROM VECTOR HIS B 102 CLONING ARTIFACT MUTATION ARISING FROM VECTOR MET B 103 CLONING ARTIFACT MUTATION ARISING FROM VECTOR
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR details
Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED PROTEIN. DOUBLE-HALF FILTERED 2D NOESY AND HALF-FILTERED HSQC-NOESY ON A MIXED LABELED/UNLABELED SAMPLE ...Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED PROTEIN. DOUBLE-HALF FILTERED 2D NOESY AND HALF-FILTERED HSQC-NOESY ON A MIXED LABELED/UNLABELED SAMPLE WERE USED TO DISTINGUISH INTRA- AND INTER-MONOMER NOES. RESIDUES 102-109 AND 171 ARE INSUFFICIENTLY CONSTRAINED BY THE INPUT DATA AND SHOULD BE CONSIDERED OF UNKNOWN STRUCTURE. THUS THE STRUCTURE IS ONLY DEFINED FOR RESIDUES 110 - 170.
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Sample preparation
Details
Contents: 0.6-1.4 MM MOUSE HP1 C-TERMINAL DOMAIN (AS MONOMER), 10 MM SODIUM PHOSPHATE BUFFER, 10 MM PERDEUTERATED DTT, 0.05% SODIUM AZIDE
Sample conditions
pH: 8.0 / Pressure: 1 atm / Temperature: 303 K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker AMX
Bruker
AMX
500
1
Bruker AVANCE
Bruker
AVANCE
600
2
Bruker AVANCE
Bruker
AVANCE
800
3
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Processing
NMR software
Name
Version
Developer
Classification
CNS
0.9
BRUNGERETAL
refinement
Azara
structuresolution
ANSIG
structuresolution
CNS
structuresolution
ARIA
structuresolution
Refinement
Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. STRUCTURE WAS GENERATED FROM EXPERIMENTS CARRIED OUT AT 303-308K WITH PH 7.5-8.0
NMR ensemble
Conformer selection criteria: LEAST NOE VIOLATION ENERGY / Conformers calculated total number: 35 / Conformers submitted total number: 16
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